中国组织工程研究2017，Vol.21Issue(9)：1324-1328,5.DOI:10.3969/j.issn.2095-4344.2017.09.003
基于"单细胞移植-成瘤性验证"模式鉴定肝癌干细胞
A preliminary establishment of the"single-cell-transplant-tumorigenic-test"model for identifying liver cancer stem cells
摘要
Abstract
BACKGROUND: There is no effective method to identify liver cancer stem cells until now, which has become one of the major challenges in this field. OBJECTIVE: To explore a more effective and suitable way for the identification of liver cancer stem cells in hepatocellular carcinoma cell lines. METHODS: Firstly, the single cell separation technique was used to obtain single cells, which were seeded into 96-wel plates one by one. Secondly, cell sublines derived from single cell colonies (tumorigenic colonies) were selected and obtained. At last, the cells from these clones were transplanted into the forelimb armpits of nude mice to observe the tumorigenic ability. RESULTS AND CONCLUSION: The number of holes of single cells obtained was 371 by the single cell separation technique, and the success rate was 96.4%. According to the growth of cell clone, tumorigenic colonies were selected to be transplanted to the forelimb armpits of nude mice, and the tumor formation rate of the colonies was 100%. The identification model of "single-cell-transplant-tumorigenic-test" for liver cancer stem cells is confirmed to be preliminarily established, which lays the technology and methodological basis for the follow-up research.关键词
干细胞/肿瘤干细胞/单细胞移植-成瘤性/肝癌干细胞/干细胞鉴定/HepG2肝癌细胞/裸鼠/细胞移植/国家自然科学基金分类
医药卫生引用本文复制引用
贾微,岑妍慧,包鹃,杨瑞,何国珍,吴晓君,李熠毅..基于"单细胞移植-成瘤性验证"模式鉴定肝癌干细胞[J].中国组织工程研究,2017,21(9):1324-1328,5.基金项目
国家自然科学基金项目(81503406) （81503406）
广西高校科研课题(ZD2014068) （ZD2014068）
广西高校青年教师能力提升计划(KY2016YB218, KY2016YB224) （KY2016YB218, KY2016YB224）
广西壮族自治区卫生厅中医药科技专项课题(GZPT13-04 , GZBZ16-07,GZLC16-23),广西中医药大学学生科研训练课题(2014DXS02) the National Natural Science Foundation of China, No. 81503406 （GZPT13-04 , GZBZ16-07,GZLC16-23）
University Research Projects in Guangxi, No. ZD2014068 （）
Project of Improving the Basic Ability of Young Teachers in Universities in Guangxi, No. KY2016YB218, KY2016YB224 （）
Special Scientific Project of Traditional Chinese Medicine supported by Health Department of Guangxi Zhuang Autonomous Region, No. GZPT13-04, GZBZ16-07, GZLC16-23 （）
and Student Research Training Project of Guangxi Medical University, No. 2014DXS02 （）
