中国组织工程研究2017，Vol.21Issue(9)：1329-1333,5.DOI:10.3969/j.issn.2095-4344.2017.09.004
结直肠癌组织中CD44+/SOX2+肿瘤干细胞的分布及形态特征
Distribution and morphological characteristics of CD44+/SOX2+cancer stem cells in colorectal carcinoma
袁杭 1刘斌 2铁娅滕 1孙亚敏 2辛晴 3苏勤军 4李双明1
作者信息
- 1. 解放军兰州军区兰州总医院病理科,甘肃省兰州市 730050
- 2. 兰州大学基础医学院病理研究所,甘肃省兰州市 730030
- 3. 甘肃省干细胞与基因药物重点实验室,甘肃省兰州市 730050
- 4. 甘肃中医药大学基础医学院,甘肃省兰州市 730000
- 折叠
摘要
Abstract
BACKGROUND:CD44 is commonly used as a marker of colorectal cancer stem cells. In recent years, it is found that transcription factor SOX2 is a potential marker of colorectal cancer stem cells. OBJECTIVE:To observe the number, distribution and morphological characteristics of CD44+/SOX2+cells in colorectal cancer, and to explore whether CD44+/SOX2+is a marker of colorectal cancer stem cells. METHODS:Immunehistochemical double staining method was used to observe the number and distribution of CD44+/SOX2+cells in 10 cases of normal intestinal mucosa, 20 cases of colorectal adenoma and 88 cases of cancer tissue specimens. Hematoxylin-eosin staining was used to observe the morphological characteristics of CD44+/SOX2+cells in colorectal cancer tissues. RESULTS AND CONCLUSION: Immunehistochemical double staining showed that: there were no CD44+/SOX2+cells in normal intestinal mucosa, a very small amount of CD44+/SOX2+cells in colorectal adenoma, and a small amount of CD44+/SOX2+cells in colorectal carcinoma. The CD44+/SOX2+cells in highly and moderately differentiated adenocarcinoma were scattered or distributed focally in the basal part of crypt or along the co-walled gland. In poorly differentiated adenocarcinoma, they were scattered or distributed focally in other cancer cells. Hematoxylin-eosin staining showed that the CD44+/SOX2+cells were small, round or oval structure, with less cytoplasm, large nuclear, obvious nucleolus, and the mitotic phenomenon was rarely seen. There was no correlation between the expression of CD44+/SOX2+and age, gender, location, depth of invasion in colorectal cancer patients;there was a negative correlation with differentiation and a positive correlation with TNM staging, distant metastasis. To conclude, CD44+/SOX2+cells are in line with the characteristics of tumor stem cells in terms of distribution and morphology character, and CD44+/SOX2+may be a marker of colorectal cancer stem cells.关键词
干细胞/肿瘤干细胞/结直肠癌/CD44/SOX2/组织芯片/免疫组织化学双重染色分类
医药卫生引用本文复制引用
袁杭,刘斌,铁娅滕,孙亚敏,辛晴,苏勤军,李双明..结直肠癌组织中CD44+/SOX2+肿瘤干细胞的分布及形态特征[J].中国组织工程研究,2017,21(9):1329-1333,5.
