中国组织工程研究2017，Vol.21Issue(9)：1352-1356,5.DOI:10.3969/j.issn.2095-4344.2017.09.008
低氧下血管内皮细胞生长因子转染人骨髓间充质干细胞向血管内皮样细胞的分化
Vascular endothelial growth factor transfection induces human bone marrow mesenchymal stem cells differentiating into endothelial-like cells under hypoxia
摘要
Abstract
BACKGROUND: It has been found that vascular endothelial growth factor can induce the differentiation of bone marrow mesenchymal stem cells into endothelial cells, but can the vascular endothelial growth factor gene promote the differentiation of bone marrow mesenchymal stem cells into vascular endothelial cells in the damaged organ under the hypoxic environment? OBJECTIVE: To observe whether human bone marrow mesenchymal stem cells induced by vascular endothelial growth factor could differentiate into vascular endothelial cells under hypoxia. METHODS: The third passage of human bone marrow mesenchymal stem cells were cultured in vitro. Cells in the control group were cultured with conventional culture medium, while those in experimental group were cultured with adenovirus vector containing vascular endothelial growth factor in 5% O2. After 2 weeks of culture, morphological observation and surface-related molecular detection were performed. The levels of vascular endothelial growth factor and endothelial nitric oxide synthase were detected by ELISA. The expression of endothelin and prostacyclin was detected by RT-PCR and western blot assay. RESULTS AND CONCLUSION: (1) The number of cells in the control group was more than that in the experimental group. The cells in the control group were crowded and arranged irregularly, showing a fiber-like growth, while those in the experimental group were mostly triangular or polygonal, exhibiting a colony-like growth. (2) CD31 was negative in the control group, while CD105 was positive and the positive rate was 99.7%, indicating that the cells still showed the phenotype of bone marrow mesenchymal stem cells. The positive rate ofCD31 was significantly increased to 30.33% in the experimental group and the positive rate of CD105 expression was decreased to 58.11%, indicating a typical phenotype of endothelial cells. (3) Compared with the control group, the expression of endothelin, vascular endothelial growth factor and endothelial nitric oxide synthase increased significantly in the experimental group (P < 0.05), and the expression of prostacyclin decreased significantly (P < 0.05). All these findings indicate that vascular endothelial growth factor can promote the differentiation of human bone marrow mesenchymal stem cells into vascular endothelial cells under hypoxia.关键词
干细胞/骨髓干细胞/血管内皮细胞生长因子/骨髓间充质干细胞/血管内皮细胞/内皮型一氧化氮合成酶/内皮素/前列环素分类
医药卫生引用本文复制引用
胡继宏,贾佳,路娟,王秋萍,赵静苗,靳利梅,李金娟..低氧下血管内皮细胞生长因子转染人骨髓间充质干细胞向血管内皮样细胞的分化[J].中国组织工程研究,2017,21(9):1352-1356,5.基金项目
甘肃省高校基本科研业务费项目(2012-3) the Fundamental Research Fund for Gansu Universities, No. 2012-3 （2012-3）
