中国组织工程研究2017，Vol.21Issue(9)：1373-1377,5.DOI:10.3969/j.issn.2095-4344.2017.09.012
树鼩脐带间充质干细胞分离、培养及成骨成脂分化的鉴定
Tree shrew umbilical cord mesenchymal stem cells:isolation, cultivation and osteogenic and adipogenic differentiation
摘要
Abstract
BACKGROUND: Studies have shown that umbilical cord mesenchymal stem cells are ideal seed cells for tissueengineering research.OBJECTIVE: To isolate, culture and identify tree shrew umbilical cord mesenchymal stem cells, in order toestablish a standardized tree shrew umbilical cord mesenchymal stem cell lines.METHODS: Caesarean-isolated tree shrew umbilical cord samples were used to isolate and culture umbilical cordmesenchymal stem cells using tissue explant adherent method. Flow cytometry assay was used to detect cellsurface markers. Osteogenic and adipogenic induction media were used to induce umbilical cord mesenchymalstem cells to differentiate into osteoblasts and adipocytes.RESULTS AND CONCLUSION: The cultured umbilical cord mesenchymal stem cells expressed CD90 and CD105 with the positive rate of 99.9% and 99.8% respectively. Hematopoietic stem cell marker CD34 expression ratewas 0.0% and the endothelial cell marker CD31 expression rate was 0.7%, in line with the characteristics of umbilicalcord mesenchymal stem cell surface markers. Calcium nodules by alizarin red staining and lipid droplets by oil red Ostaining were observed in the induced cells. These experimental findings indicated that umbilical cord mesenchymalstem cells from tree shrews capable of osteogenic and adipogenic differentiation were successfully isolated and cultured.关键词
干细胞/脐带脐血干细胞/脐带间充质干细胞/树鼩/成骨分化/成脂分化/CD抗原/表面标志物/钙结节/脂滴/分离培养/种子细胞分类
医药卫生引用本文复制引用
阮光萍,朱路,刘菊芬,李自安,王金祥,庞荣清,潘兴华..树鼩脐带间充质干细胞分离、培养及成骨成脂分化的鉴定[J].中国组织工程研究,2017,21(9):1373-1377,5.基金项目
国家科技支撑计划项目(2014BAI01B01) （2014BAI01B01）
云南省科技计划项目(2013CA005) the National Support Program of China, No. 2014BAI01B01 （2013CA005）
Yunnan Provincial Science and Technology Project, No. 2013CA005 （）
