中国组织工程研究2017，Vol.21Issue(9)：1397-1401,5.DOI:10.3969/j.issn.2095-4344.2017.09.016
碱性成纤维细胞生长因子基因修饰骨骼肌卫星细胞自体移植急性心肌梗死组织的血管新生
Effects of basic fibroblast growth factor modified skeletal muscle satellite cell transplantation on neovascularization following acute myocardial infarction
摘要
Abstract
BACKGROUND: Previous studies have found that skeletal muscle satellite cell transplantation can induce angiogenesisin myocardial infarction area, reduce infarct size and improve cardiac function. But the overall effect is not satisfactory.OBJECTIVE: To observe the survival of basic fibroblast growth factor (bFGF) gene modified skeletal muscle satellitecells in acute myocardial infarction and to observe the expression of bFGF gene and the effect of cell transplantation onangiogenesis in myocardial infarction area.METHODS: Eighteen New Zealand white rabbits were divided into three groups by random: skeletal muscle satellite cellgroup (control group), bFGF gene enhanced skeletal muscle satellite cell group (experimental group) and blank controlgroup. The left anterior descending branch of the coronary artery of the rabbits was ligated so as to establish an animalmodel of acute myocardial infarction in the former two groups. After labeled by DAPI before transplantation, the skeletalmuscle satellite cells, bFGF gene modified skeletal muscle satellite cells and the equivalent amount of DMEM/F12 wereinjected into the local infarct myocardium correspondingly. Samples were taken 4 weeks after transplantation. Then, thesurvival of skeletal muscle satellite cells and the expression of bFGF gene were observed under light microscope andfluorescence microscope, and the neovascularization in the myocardial infarction area was examined byimmunohistochemical staining.RESULTS AND CONCLUSION: No DAPI-labeled cells were visible in the blank control group, but in the other twogroups, a large amount of DAPI-labeled skeletal muscle satellite cells were seen in the infarction area. Enhanced greenfluorescent protein was highly expressed in the experimental group. Microvessel density in the infarction area washighest in the experimental group followed by the control and blank control groups (P < 0.05). These findings indicatethat bFGF gene modified skeletal muscle satellite cells can survive and promote neovascularization in the acutemyocardial infarction area.关键词
干细胞/移植/干细胞移植/骨骼肌卫星细胞/骨骼肌干细胞/碱性成纤维细胞生长因子/基因修饰/心肌梗死/血管生成分类
医药卫生引用本文复制引用
许志锋,李敬来,韩振,冯钢,任明明..碱性成纤维细胞生长因子基因修饰骨骼肌卫星细胞自体移植急性心肌梗死组织的血管新生[J].中国组织工程研究,2017,21(9):1397-1401,5.基金项目
深圳市科技计划项目(200404104) the Science and Technology Project of Shenzhen City, No. 200404104 （200404104）
