中国组织工程研究2017，Vol.21Issue(9)：1408-1413,6.DOI:10.3969/j.issn.2095-4344.2017.09.018
滑膜间充质干细胞与软骨细胞移植修复膝关节软骨缺损
Repairing articular cartilage defect of the knee joint by synovial mesenchymal stem cells and chondrocytes
摘要
Abstract
BACKGROUND: As is known to all, it is difficult to repair cartilage defect of the knee joint. Fortunately, it isreported that a chitosan scaffold with the mixture of synovial mesenchymal stem cells and chondrocytes enablesthe formation of cartilage matrix.OBJECTIVE: To observe the chondrogenic differentiation of synovial mesenchymal stem cells co-cultured withchondrocytes on a chitosan/type Ⅰ collagen composite scaffold after being transplanted into an animal model. METHODS: The synovial mesenchymal stem cells and chondrocytes harvested from the knee joint of Sprague-Dawleyrats were co-cultured at a ratio of 1:2 on the chitosan/type Ⅰ collagen composite scaffolds and transplanted into the defectsite of the knee joint in rat models of cartilage defect of the knee joint.RESULTS AND CONCLUSION: After 4, 8, 12 weeks, the hematoxylin-eosin staining, the toluidine blue staining ofaggrecan, and safranine O staining showed that there were increasing chondrocytes in the rat knee joint. Theimmunohistochemical staining of type Ⅱ collagen showed the positive expression of type Ⅱ collagen in the cartilagetissues and extracellular matrix of the knee joint. To conclude, the combination of two kinds of cells cultured in thechitosan/type Ⅱ collagen composite scaffold can form cartilage-like tissues in vivo, and improve the quality of cartilagerepair.关键词
干细胞/移植/干细胞移植/软骨组织工程/滑膜间充质干细胞/软骨细胞/共培养技术/Ⅱ型胶原/蛋白聚糖/国家自然科学基金分类
医药卫生引用本文复制引用
胡鑫,王玥,林兆全,尹小朋,龚忠诚,邵博,凌彬,刘慧,克热木•阿巴斯,王冰,胡露露..滑膜间充质干细胞与软骨细胞移植修复膝关节软骨缺损[J].中国组织工程研究,2017,21(9):1408-1413,6.基金项目
国家自然科学基金项目(31260229) （31260229）
新疆维吾尔自治区青年科技创新人才培养工程项目(2014721046) the National Natural Science Foundation of China, No. 31260229 （2014721046）
the Scientific Innovation Talent Training Project of Xinjiang Uygur Autonomous Region of China, No. 2014721046 （）
