新疆农业科学2017，Vol.54Issue(2)：213-222,10.DOI:10.6048/j.issn.1001-4330.2017.02.003
沉默茉莉酸甲基转移酶(GhJMT)基因对棉花幼苗生理指标的影响
Silencing Effect of Jasmonic Acid Carboxyl Methyl Transferase(JMT)Gene on Physiological Indexes of Cotton Seedlings
摘要
Abstract
[Objective] Jasmonic methylester transferase(JMT)can effectively facilitate the production of jasmonic methylester,so that it enhances plant drought resistance.[Method]A 450 bp silence fragment of GhJMT was cloned from cotton XLZ17(Gossypium hirsutum)using PCR amplification,and then constructed into vector pTRV.The recombinant plasmid pTRV-GhJMT was transformed into competent Agrobacterium tumefaciens(GV3101).Cotton seedlings in hydroponic conditions were infected with the transformed A.tumefaciens GV3101.After one month,the growing cotton seedlings were treated with PEG6000 for 24 h.The water content,the molality of osmotic adjustment substances,photosynthetic pigments,antioxidants and malondialdehyde(MDA)as well as enzymes activities in cotton leaves under drought stress were measured.[Result]The results showed that content of soluble sugar,ASA,betaine,proline and SOD were reduced by 42.1％,36.6％,69.4％and 71.6％compared with the pTRV-00,and MDA,O2-and H2O2 were increased 0.43,1.45 and 0.59 times compared with pTRV-GhJMT,respectively.[Conclusion]Our results demonstrate that GhJMT gene silencing can directly affect the physiological and biochemical characteristics of cotton seedlings treated with drought stress and reduce drought resistant ability of cotton.关键词
棉花/干旱胁迫/VIGS/生理生化特性/基因表达Key words
Gossypium hirsutum/drought stress/virus-induced gene silencing (VIGS)/physiological and biochemical characteristics/gene expression分类
农业科技引用本文复制引用
安汶铠,严晓红,包秋娟,张富春..沉默茉莉酸甲基转移酶(GhJMT)基因对棉花幼苗生理指标的影响[J].新疆农业科学,2017,54(2):213-222,10.基金项目
国家自然科学基金-新疆联合基金重点项目"棉花水分高效利用的关键基因的挖掘及分子机理的研究"(U1303282) Joint key fund of National Natural Science Foundation of China and Xinjiang "Exploring key genes related to high-efficiency water utilization and creating of drought resistant materials in cotton" (U1303282) （U1303282）
