新疆农业科学2017，Vol.54Issue(2)：223-233,11.DOI:10.6048/j.issn.1001-4330.2017.02.004
利用SSR标记对新疆部分向日葵自交系材料进行群体结构划分及聚类分析
Study on the Population Structure and Cluster Analysis of Some Sunflower Inbred Lines in Xinjiang by SSR Markers
摘要
Abstract
[Objective] Sequence Tagged Site(STS)is a short DNA sequence,which appears only once on its biological chromosome and is suitable for a landmark of crop genome.[Method]500 STS-SSR primers of sunflower were synthesized randomly and were used to screen the samples of Xinjiang edible sunflower and oil sunflower inbred lines for finding the polymorphism.[Result]All of them 62 markers showed stable polymorphism among those 136 sunflowers with clear,stable and easy to read characteristics,which accounted for 12.4％of the number of selected primers.136 Xinjiang sunflower inbred populations could be divided into 2 groups according to the change of △K and be clustered into 2 groups at the 0.65 similarity level.In the 73 oil sunflower inbred lines,70％inbred lines were assigned to first groups which meant the oil sunflower inbred lines were mainly classified in the first group.Among 30 maintainer lines of oil sunflower lines only 4 maintainer lines were assigned to second groups,the rest were in group I.In the 43 restorer line of oil sunflower,25 restorer lines were allocated to first large groups,and the rest were allocated to other groups,which indicated that the genetic diversity of restorer line was more abundant than maintainer lines.In the 55 edible sunflower inbred lines,only 21％of them were divided into first groups which indicated that edible sunflower inbred lines belonged to the second and the third groups.[Conclusion]This study showed that the genetic diversity of edible sunflower was greater than that of oil sunflower,and some genes between the edible sunflower and oil sunflower materials were crossed and penetrated.The results of this study have provided theoretical support for the construction of the core collection and the rational utilization of resources in the future.关键词
向日葵/序列标签位点/群体结构/聚类分析Key words
sunflower/sequence tagged site/population structure/cluster analysis分类
农业科技引用本文复制引用
刘胜利,段维,王鹏,柳延涛,王沛政..利用SSR标记对新疆部分向日葵自交系材料进行群体结构划分及聚类分析[J].新疆农业科学,2017,54(2):223-233,11.基金项目
兵团科技支疆项目"向日葵快速高效选育技术及种质资源创新研究"(2014AB007) （2014AB007）
国家向日葵现代产业技术体系建设项目(CARS-16) The Yuanjiang project of Science and technology Corps"Study on breeding techniques with rapid and efficient and Germplasm Resources Innovation of sunflower" (2014AB007) （CARS-16）
The project of national Industrial technology system of sunflower (CARS-16) （CARS-16）
