新疆农业科学2017，Vol.54Issue(2)：262-271,10.DOI:10.6048/j.issn.1001-4330.2017.02.008
天山云杉成熟与未成熟合子胚的胚性愈伤组织诱导及增殖研究
Study on the Induction and Proliferation of Somatic Embryogenesis from the Mature and Immature Zygote Embryos of Picea schrenkiana
摘要
Abstract
[Objective] To study the induction and proliferation of somatic embryogenesis from the mature and immature zygote embryos of Picea schrenkiana,which might have great significance for the understanding of the mechanism of embryonic development and the establishment of rapid propagation system.[Method]Immature embryo and mature embryo were used as explants to induce culture in DCR medium with different growth regulator concentrations.The induction rates of zygotic embryos in different growth stages and its proliferation were studied using immature zygotic embryos with endosperm,immature zygotic embryos without endosperm and mature embryos as explants.[Result](1)When DCR medium added with different concentrations of 2,4-D and 6-BA was used to induce mature zygotic embryos,the combination of 20 μM 2,4-D and 5 μM 6-BA was the best and somatic embryogenesis induction rate was the highest,up to 55％.(2)The induction rate of three zygotic embryos of somatic embryogenesis collected in different periods showed that the immature zygotic embryos with endosperm(June)was up to 32.6％,the mature zygotic embryos(September)was up to 60.6％,Immature embryo without endosperm(July)was 83.1％,which indicated that July was the best picking period of P.schrenkiana cones.(3)The somatic embryogenesis in the five different proliferation medium showed the the proliferation rate was significant(P< 0.05).The proliferation results of somatic embryogenesis in different proliferation medium showed that the effect of BM2 and SH were better than the MSG,1/2DCR,DCR,and the comprehensive proliferation effect of SH was the best.The proliferation rate was 4.55 g and the rate was up 600％.)(4)The five different somatic embryogenesis in SH proliferation medium showed that the difference between somatic embryogenesis was not significant(P > 0.05).[Conclusion]This study has established the somatic embryogenesis technology platform of P.schrenkiana for the first time,which has provided a scientific basis for the genetic improvement of germplasm and shortened the breeding cycle of P.Schrenkiana.关键词
天山云杉/培养基/生长调节剂/胚性愈伤组织Key words
Picea schrenkiana/culture media/plant growth regulator/somatic embryogenesis分类
农业科技引用本文复制引用
伊丽米努尔,李宏..天山云杉成熟与未成熟合子胚的胚性愈伤组织诱导及增殖研究[J].新疆农业科学,2017,54(2):262-271,10.基金项目
新疆维吾尔自治区自然科学基金项目"天山云杉体细胞胚胎发育的相关研究"(2013211A036) Supported by Natural Science Fund of Xinjiang Uygur Autonomous Region"Related research on the somatic embryogenesis of Picea schrenkiana"(2013211A036) （2013211A036）
