新疆农业科学2017，Vol.54Issue(2)：313-319,7.DOI:10.6048/j.issn.1001-4330.2017.02.014
枣实蝇气味结合蛋白基因的克隆及表达谱分析
Cloning and Expression Profiling of Odorant Binding Protein Gene in Carpomya vesuviana (Costa) (Diptera: Tephritidae)
摘要
Abstract
[Objective] This study aims to clone Carpomya vesuviana Costa odorant binding protein(CvcoOBP)and analyze expression profile of CvcoOBP in order to lay the foundation of researching CvcoOBP in Carpomya vesuviana Costa.[Method]Based on transcriptome sequencing of cephalic RNA,the study has analyzed CvcoOBP according to alignment and phylogenetic analysis.The research was also conducted into the tissue specific expression of CvcoOBP by semi-quantitative reverse transcription and polymerase chain reaction(sqRT-PCR).[Result]The study has obtained seven CvcoOBP that had been named respectively as CvcoOBP1-7(GenBank accession number: KU975053,KU975054,KU975055,KX059394,KU975056,KX059395 and KX059396).According to amino acid alignment,CvcoOBP1,CvcoOBP3,CvcoOBP4,CvcoOBP6 and CvcoOBP7 had been proved to be a family of classic OBP,which contained six highly conversed cysteine residues.CvcoOBP2 and CvcoOBP5 belonged to a family of "Minus-C",which had only four highly conversed cysteine residues.Phylogenetic analysis showed that CvcoOBP1-7 had close relationships with different OBPs.Finally,sqRT-PCR revealed that CvcoOBP1-7 could be highly expressed in abdomen,head and thorax.CvcoOBP1 and CvcoOBP2 could be also expressed in wings with relatively low expression.CvcoOBP1,CvcoOBP5 and CvcoOBP6 were also found in legs.[Conclusion]For the first time,the study obtained and analyzed seven CvcoOBP.Different CvcoOBP are various in terms of expression sites and expression quantity.The CvcoOBP1-6 separately belongs to OBP56g,OBP8a,OBP83a,OBP56h,OBP99c and OBP83ef type.关键词
枣实蝇/气味结合蛋白/组织表达Key words
Carpomya vesuvianaCosta/odorant binding protein/expression profiling分类
农业科技引用本文复制引用
李亚伟,张小菊,王科珂,于艳雪,张祥林,张伟,张俊华,杨定,陈乃中..枣实蝇气味结合蛋白基因的克隆及表达谱分析[J].新疆农业科学,2017,54(2):313-319,7.基金项目
国家质检总局科研项目"重要检疫性入侵害虫枣实蝇监测诱集物质"(2015IK321) （2015IK321）
国家"十二五"科技支撑计划"重要外来有害生物传入风险及监测技术"(2015BAD08B01) （2015BAD08B01）
Research projects of the State Administration of quality supervision and inspection"Monitoring attractants for important quarantine pest of Carpomya vesuviana Costa"(2015IK321) （2015IK321）
National Science and technology support program in 12th Five-Year"Study on the risk and monitoring technology for the introduction of invasive pests"(2015BAD08B01) （2015BAD08B01）
AQSIQ Non-Profit Industry Research Projects "Research on the colonization factors and risk factors of alien organisms and their control techniques"(201410014). （201410014）
