新疆农业科学2017，Vol.54Issue(2)：361-370,10.DOI:10.6048/j.issn.1001-4330.2017.02.020
盐胁迫下盐穗木DNA聚合酶λ基因的克隆和表达分析
Cloning and Expression Analysis of HcDNA pol λ Gene from Halostachys caspica under Salt Stress
摘要
Abstract
[Objective] The expression of DNA damage repair gene under salt stress is helpful to reveal the relationship between DNA damage repair and salt tolerance of halophyte.[Method]According to the transcriptome of Halostachys caspica under salt stress,a DNA damage repair gene DNA polymerase lambda(HcDNA pol λ)was cloned from Halostachys caspica by RACE.[Result]Sequence analysis indicated that HcDNA pol λ contained an open reading frame of 1,335 bp,which encodes 444 amino acids.Conserved domain analysis showed that HcDNA pol λ was the number of DNA polymerase X family,and phylogenetic tree analysis indicated that HcDNA pol λ was an independent branch,which was an stable hydrophilic proteins sub-celled nuclear.Real time fluorescent quantitative PCR analysis showed that the expression of HcDNA pol λ of assimilating branches gradually up-regulated with the increase of NaCl concentration.At 300 mmol/L NaCl treatments,the expression levels of HcDNA pol λ were increased 3 fold and 5 fold for 7 days and for 14 days,respectively.Finally,at 700 mmol/L NaCl treatment for 14 days,the expression levels of HcDNA pol λ increased 20 fold and reached the peak.[Conclusion]The expression of HcDNA pol λ gene could be induced by salt stress.关键词
盐穗木/HcDNApolλ基因/盐胁迫/基因表达Key words
Halostachys caspica/HcDNA pol λ gene/salt stress/expression analysis分类
农业科技引用本文复制引用
张冀,杜驰,张富春..盐胁迫下盐穗木DNA聚合酶λ基因的克隆和表达分析[J].新疆农业科学,2017,54(2):361-370,10.基金项目
新疆重点实验室专项资金"盐穗木盐胁迫响应的转录组研究及重要基因的功能鉴定"(2014KL001) （2014KL001）
国家"973"计划前期研究专项"新疆荒漠盐生植物耐盐的生理及分子机制"(2012CB722204) Special funds of Xinjiang Key Laboratory "The salt-stress responsive transcriptome of the halophyte Halostachys caspica and the functional identification of the major salt related genes"(2014KL001) and the National 973 Pre-Research Project "Physiological and molecular mechanisms of salt tolerance of desert halophytes in Xinjiang"(2012CB722204) （2012CB722204）
