畜牧与兽医2017，Vol.49Issue(3)：47-51,5.
鸡血藤总黄酮对脂多糖刺激RAW264.7细胞氧化应激的调节作用
Effect of total flavonoids of Spatholobus suberectus Dunn on LPS-induced oxidative stress in RAW264.7 cells
摘要
Abstract
Total flavonoids of Spatholobus suberectus Dunn (FSSD) was obtained by ethanol extraction.The cytotoxicity of FSSD was analyzed using MTT assay.Its anti-oxidative stress activity was evaluated by both pre-treatment and post-treatment of RAW264.7 cells under oxidative stress induced by lipopolysacchride (LPS) with FSSD.For pre-treatment,RAW264.7 cells were treated with series concentration (25,50 and 100 μg/mL) of FSSD for 12 h before LPS stimulation.For post-treatment,RAW264.7 cells were firstly stimulated by LPS for 2 h and then treated with series concentration of FSSD for 8 h.NO and reactive oxygen species (ROS) levels were analyzed using Griess assay and fluorescent probe label method.It was found that both FSSD pre-treatment and post-treatment can inhibit the production of NO and ROS induced by LPS,and post-treatment showed better effect compared to pre-treatment method.Thus,FSSD has high anti-oxidative stress activity and it is more suitable to be used for treatment of oxidative stress rather than prevention.关键词
鸡血藤/总黄酮/脂多糖/氧化应激/细胞毒性Key words
total flavonoids/Spatholobus suberectus Dunn/lipopolysacchride/oxidative stress/cytotoxicity分类
农业科技引用本文复制引用
赵尉丹,蒙西南,付远妨,胡庭俊,黄诗琦,陈海兰..鸡血藤总黄酮对脂多糖刺激RAW264.7细胞氧化应激的调节作用[J].畜牧与兽医,2017,49(3):47-51,5.基金项目
广西自然科学基金(2014GXNSFCA118019)、广西教育厅科研项目(KY2015ZD007)、“大学生创新创业训练计划”创新训练项目(201610593081) （2014GXNSFCA118019）
