畜牧与兽医2017，Vol.49Issue(3)：72-77,6.
基于血清代谢组学研究Kisspeptin对热应激大鼠的保护作用
Study on the protective effects of Kisspeptin in rats after heat stress by serum metabonomic approach
张征 1雷治海 1贾翠翠 1李晓亮 1侯元龙 1索川 1张莹 1苏娟1
作者信息
- 1. 南京农业大学动物医学院,江苏南京210095
- 折叠
摘要
Abstract
The protective effects of Kisspeptin in rats after heat stress were investigated using metabonomic method.Gas chromatography coupled to time-of-flight mass spectrometry (GC/TOFMS) was used to detect the serum endogenous metabolites in rats.Serum metabolite profiles were analyzed with multivariate statistical analysis,such as principal component analysis (PCA) and partial least squares-discriminate analysis (PLS-DA).The result showed that a clear difference was observed among control,heat stress and heat stress treated with Kisspeptin groups.The differentially expressed metabolites and potential biomarkers were selected and identified according to parameters of variable importance in the projection (VIP) and one-way ANOVA.Results showed that the serum metabolic profiling of heat stress-induced rats was significantly different from that of control group,and it had the tendency to the normal state after Kisspeptin treatment.The metabolic disorders of TCA cycle,saccharometabolism,amino acid,and nucleoside metabolism were induced by heat stress based on biochemical metabolic pathway analysis.However,the metabolic disorders were rescued after Kisspeptin treatment.The oxidative stress parameters analysis showed that Kisspeptin treatment significantly inhibited malondialdehyde (MDA) level,and restored superoxide dismutase (SOD),total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px) activities.This study provides the experimental basis for healthy breeding and the development of safe biological products for anti-heat stress.关键词
热应激/代谢组学/大鼠/血清/氧化应激Key words
heat stress/metabonomic/rat/serum/oxidative stress分类
农业科技引用本文复制引用
张征,雷治海,贾翠翠,李晓亮,侯元龙,索川,张莹,苏娟..基于血清代谢组学研究Kisspeptin对热应激大鼠的保护作用[J].畜牧与兽医,2017,49(3):72-77,6.
