畜牧与兽医2017，Vol.49Issue(3)：92-95,4.
奶牛泰勒虫病的诊断及病原体18S rDNA分析
Diagnosis of theileriosis and 18S rDNA analysis of pathogen in cows
摘要
Abstract
A cow suffering a suspected blood protozoosis was diagnosed by conventional blood smear microscopy and PCR techniques in Tacheng,Xinjiang.The 18S rRNA gene was amplified using universal primers and cloned.The sequence identities were analyzed with among the 18S rRNA gene sequences of Theileria and Babesia reference strains from GenBank,and phylogenetic tree was constructed to determine the parasite species.The results of blood smear test showed that circular or oval parasites with the typical characteristics of bovine Theileria annulata were found in red blood cells.Identification of molecular biology showed that 18S rRNA gene of Tacheng strain shared 99％ identities with the nucleotide sequences of T.Annulata from French and southern Europe.Phylogenetic analysis based on 18S rRNA genes confirmed that the Tacheng strain has close relationship with the French strain and southern Europe strain of T.annulata.The result indicated that the infected species in the cow was confirmed to be T.annulata.关键词
环形泰勒虫/同源性/18S rRNA基因/系统进化树Key words
Theileria annulata/identities/18S rRNA gene/phylogenetic tree分类
农业科技引用本文复制引用
于伟伟,孟庆玲,乔军,卢海亭,贡莎莎,程子兵,张金生,才学鹏,陈创夫..奶牛泰勒虫病的诊断及病原体18S rDNA分析[J].畜牧与兽医,2017,49(3):92-95,4.基金项目
兵团中青年科技创新领军人才计划(2016BC001) （2016BC001）
国家公益性(农业)行业专项(201303037) （农业）
