新医学2017，Vol.48Issue(2)：87-93,7.DOI:10.3969/j.issn.0253-9802.2017.02.005
VIP 通过 VPAC1／AKT 通路抑制溃疡性结肠炎的肠道炎症反应
VIP inhibits the intestinal inflammation in ulcerative colitis through VPAC1/AKT signaling pathway Xu
摘要
Abstract
Objective To investigate the role of vasoactive intestinal peptide (VIP)and VPAC1 /AKT signaling pathway in ulcerative colitis (UC).Methods The colon mucosal tissues were collected from the UC patients and the expression of VIP and its receptor VPAC1 were detected.All mice were divided to three groups.In the acute UC group,mouse models with acute UC were established by exposure to 3% dextran sulfate sodium (DSS)for 7 days.In the VIP group,each mouse was intraperitoneally injected with 1 nmol VIP on day 1 ,3,5 following 3% DSS administration.In the control group,the mice were allowed to drink distilled water.The disease activity index was recorded.The colon mucosal tissues were collected from the UC patients and mice.The changes in the expression of VIP and VPAC1 ,intestinal epithelial apoptosis and proliferation, the expression of AKT protein were observed and compared before and after VIP treatment.Results The ex-pression of VIP and VPAC1 at mRNA and protein levels were significantly down-regulated in the colon mucosal tissues of patients and mice with acute UC.After VIP administration,the disease activity index was moderately decreased,the apoptosis of the epithelial cells of colon mucosal tissues was significantly mitigated and the pro-liferation of the epithelial cells of colon mucosal tissues enhanced in the DSS-induced UC mice.The expression levels of VPAC1 and p-AKT proteins in the colon mucosa from DSS-induced UC mouse models were down-regu-lated,which were significantly up-regulated after VIP treatment.Conclusions VIP can suppress the intestinal inflammatory response,inhibit the epithelial apoptosis and promote the epithelial proliferation through VPAC1 /AKT signaling pathway in DSS-induced mouse models with acute UC.关键词
溃疡性结肠炎/血管活性肠肽/凋亡/增殖/AKTKey words
Ulcerative colitis/Vasoactive intestinal peptide/Apoptosis/Proliferation/AKT引用本文复制引用
徐敏仪,郑丰平,彭小婕,陶金,吴斌..VIP 通过 VPAC1／AKT 通路抑制溃疡性结肠炎的肠道炎症反应[J].新医学,2017,48(2):87-93,7.基金项目
广东省科技计划项目（2016A020216014）；中山大学青年教师培育项目 （）
