烟草科技2017，Vol.50Issue(1)：1-5,5.DOI:10.16135/j.issn1002-0861.2016.0373
烟草NtGGPPS4基因的克隆和功能初步分析
Cloning and functional analysis of NtGGPPS4 from Nicotiana tabacum
摘要
Abstract
To clarify the function of geranylgeranyl pyrophosphate synthase gene (NtGGPPS4) in tobacco, the coding sequence of NtGGPPS4 gene was cloned from Nicotiana tabacum. The over-expression vector pCAMBIA1300-NtGGPPS4 was constructed and transferred into Nicotiana tabacum by Agrobacterium tumefaciens mediated method. The contents of α- and β-cembrenediol and plastid pigments in transgenic tobacco plants were measured. The results showed that four transgenic tobacco plants which showed high levels of NtGGPPS4 were obtained. Comparing with the control, the contents of α- and β-cembrenediol, neoxanthin, violaxanthin, lutein, chlorophyll a, chlorophyll b and β-carotene increased significantly in transgenic plants. It indicated that NtGGPPS4 was involved in the biosynthesis of α- and β-cembrenediol and carotenoids in tobacco.关键词
烟草/牻牛儿基牻牛儿基焦磷酸合成酶基因/超量表达/功能分析Key words
Nicotiana tabacum/Geranylgeranyl pyrophosphate synthase gene/Over-expression/Functional analysis分类
轻工纺织引用本文复制引用
李锋,孟利军,史艳梅,郑庆霞,刘萍萍,魏春阳,王燃,杨军,罗朝鹏..烟草NtGGPPS4基因的克隆和功能初步分析[J].烟草科技,2017,50(1):1-5,5.基金项目
郑州烟草研究院科技项目“烟草萜类化合物合成关键基因NtGGPPS功能分析和调控机制研究”（902012CZ340）。 （）
