烟草科技2017，Vol.50Issue(1)：12-16,5.DOI:10.16135/j.issn1002-0861.2015.0668
试验土壤中烟草青枯病菌的RT-qPCR检测分析
RT-qPCR detection and quantitative analysis of Ralstonia solanacearum in soil
摘要
Abstract
To effectively control tobacco bacterial wilt, real-time quantitative PCR (RT-qPCR) technique was developed and used to quantitatively detect Ralstonia solanacearum in soil. The results showed that the lowest concentration of Ralstonia solanacearum in soil detected by the developed system was 5 × 102 cfu/g. Comparing with conventional PCR detection, the sensitivity of RT-qPCR detection to Ralstonia solanacearum in soil increased by 10 times.关键词
烟草/青枯病/土壤/青枯雷尔氏菌/RT-qPCRKey words
Tobacco/Bacterial wilt/Soil/Ralstonia solanacearum/Real-time quantitative PCR分类
农业科技引用本文复制引用
程承,李石力,刘颖,王瑢笙,丁伟,张永强..试验土壤中烟草青枯病菌的RT-qPCR检测分析[J].烟草科技,2017,50(1):12-16,5.基金项目
国家烟草专卖局重点科技项目“茄青枯病菌与烟草互作分子机制及其调控技术研究”（110201202002）。 （）
