烟草科技2017，Vol.50Issue(1)：50-57,8.DOI:10.16135/j.issn1002-0861.2016.0278
微透析-HPLC-ECD联用在线测定烟碱暴露大鼠脑内单胺神经递质的动态变化
In vivo determination of monoamine neurotransmitters in brain striatum of rat using online microdialysis coupled with high performance liquid chromatography-electrochemical detector
摘要
Abstract
To investigate potential mechanism of nicotine dependence and evaluate the monoamine neurotransmitter metabolism caused by nicotine as a function of time, online microdialysis sampling coupled with high performance liquid chromatography and electrochemical detector was developed and applied to determine the contents of dopamine (DA), 3,4-dihydroxyphenyl acetic acid (DOPAC), homovanillic acid (HVA), serotonin (5-HT) and 5-hydroxylindole acetic acid (5-HIAA) in the brain striatum of live rats after nicotine injection. Striatum dialysates were collected every 15 min at a flow rate of 2.0 μL/min and separated immediately on a HS F5 column under the gradient elution condition. The results showed that the linear range of the method were from 0.2 to 500.0 ng/mL, the recoveries ranged from 86.2% to 105.9%, while the intra-day and inter-day precisions were no more than 9.7%. In vitro microdialysate recoveries of DA, DOPAC, HVA,5-HT and 5-HIAA ranged from 25.3% to 28.3%. After intraperitoneal injection of nicotine at a rate of 2.0 mg/kg, the release of DA and 5-HT in brain striatum of rat did not increase continuously, while the release of HVA and 5-HIAA rose significantly. The method is simple, fast, sensitive and suitable for the dynamic analysis of monoamine neurotransmitters in brain of live animal administrated by nicotine via digestion.关键词
在线微透析/电化学检测/纹状体/单胺神经递质/烟碱/动态分析Key words
Online microdialysis/Electrochemical detection/Striatum/Monoamine neurotransmitter/Nicotine/Dynamic analysis分类
轻工纺织引用本文复制引用
徐妍,毛健,庆宏..微透析-HPLC-ECD联用在线测定烟碱暴露大鼠脑内单胺神经递质的动态变化[J].烟草科技,2017,50(1):50-57,8.基金项目
国家自然科学基金项目“烟碱在大鼠脑内代谢的活体动态分析及其神经递质化学效应的实时评价”（21307163）。 （）
