眼科新进展2017，Vol.37Issue(2)：110-113,4.DOI:10.13389/j.cnki.rao.2017.0029
白蒺藜对光损伤小鼠光感受器细胞的保护作用
Protective effects of tribulus terrestris L on photoreceptors from developing bright light-induced degeneration
摘要
Abstract
Objective To investigate protective effect of tribulus terrestris L (TTL) on photoreceptor in the model of light-induced retinal degeneration.Methods BALB/c mice were exposed to bright light at the intensity of 10 000 lux for 30 minutes to establish the retinal light damage models.The BALB/c mice were divided into normal control group,model group and treatment group,6 cases in each group.TTL decoction was intraperitoneally administered to mice 30 minutes prior to illumination in the treatment group.Saline vehicle was administered in the normal control group and model group.Photoreceptor protection of TTL was assessed by optical coherence tomography (OCT) at 3 hours and 7 days after illumination.Gross histology and immunohistochemistry approaches were also taken to examine the retinal protection conferred by TTL at 7 days after bright light exposure.Results Compared to normal retinal morphology in the normal control group,prominent photoreceptor loss and diminished rod and cone photoreceptors evidenced by attenuated retinal expression of rhodopsin and M-opsin were observed in the model group.In contrast,TTL treatment resulted in significant protection against bright light-induced photoreceptor degeneration and remarkable preservation of rod and cone photoreceptor cells.The outer retinal nuclear layer in the model group was thinner than that in the normal control group (P ＜ 0.05),but the treatment group was thicker than the model group (P ＜ 0.05).Conclusion Bright light induces obviously degeneration in photoreceptors in BALB/c mice.Moreover,TTL is shown for the first to significantly protect the photoreceptors from bright light-induced degeneration.关键词
白蒺藜/视网膜光损伤/光感受器细胞退行性改变Key words
tribulus terrestris L/retinal light damage/photoreceptor degeneration分类
医药卫生引用本文复制引用
徐静,卞敏娟,崔金刚,杜宵烨,张腾,陈瑜,顾江萍..白蒺藜对光损伤小鼠光感受器细胞的保护作用[J].眼科新进展,2017,37(2):110-113,4.基金项目
国家自然科学基金资助(编号:81473732) （编号:81473732）
上海市高校特聘教授(东方学者)人才计划[编号:沪教委人(2011)88号] （东方学者）
上海市085一流学科建设科技创新支持计划(编号:085ZY1221) （编号:085ZY1221）
上海市教委曙光计划(编号:13SG42)National Natural Science Foundation of China (No:81473732) （编号:13SG42）
Eastern Scholar at Shanghai Institutions of Higher Learning [No:(2011) 88] （2011）
Technological Innovation of First Class Discipline Construction of Shanghai 085 (No:085ZY1221) （No:085ZY1221）
Shuguang Project Supported by Shanghai Municipal Education Commission (No:13SG42) （No:13SG42）
