眼科新进展2017，Vol.37Issue(2)：114-116,3.DOI:10.13389/j.cnki.rao.2017.0030
一种稳定标记小鼠视网膜内两种不同类型神经节细胞的方法
A stable method to label two different types of retinal ganglion cells in mouse retina
摘要
Abstract
ObJeetive To report a simple and efficient method to label two different types of retinal ganglion cells (RGCs) in mouse retina.Methods Eyeballs were harvested from normal adult C57 BL/6 J mouse,the retinas were isolated,four radial cuts were done,the retinas were pasted on the nitrocellulose membrane with the ganglion cell layer upturned.The immunofluorescence double staining and laser confocal nmicroscope was used to reveal conventional retinal ganglion cells and intrinsically photosensitive retinal ganglion cells (ipRGCs) using Brn3a and Melanopsin.Results The double staining results of whole mount retina showed that conventional RGCs and melanopsin immunopositive ipRGCs had a complementary distribution in mouse retina,these two subtypes of RGCs were predominantly present in the ganglion cell layer.The numbers of ipRGCs was just about 1％-2％ of conventional RGCs,and the axons of ipRGCs toward the direction of the optic disc,several dendrites toward the inner plaximem layer.Conclusion The immunofluorescence double staining of whole mount retina is a simple,stable and efficient method to label two different types of mouse RGCs.关键词
小鼠/视网膜/视网膜神经节细胞/内在光敏视网膜神经节细胞Key words
mouse/retina/retinal ganglion cell/intrinsically photosensitive retinal ganglion cells分类
医药卫生引用本文复制引用
王松涛,肖虹蕾,周国民..一种稳定标记小鼠视网膜内两种不同类型神经节细胞的方法[J].眼科新进展,2017,37(2):114-116,3.基金项目
国家自然科学基金资助(编号:81170885) （编号:81170885）
新乡医学院博士科研基金资助(编号:XYBSKYZZ2015010-505119)National Natural Science Foundation of China (No:81170885) （编号:XYBSKYZZ2015010-505119）
Research Foundation for Doctors of Xinxiang Medical University (No:XYBSKYZZ2015010-505119) （No:XYBSKYZZ2015010-505119）
