眼科新进展2017，Vol.37Issue(2)：117-121,5.DOI:10.13389/j.cnki.rao.2017.0031
miRNA-146a在视网膜色素上皮细胞老化及老年性黄斑变性中的功能研究
Function of miR-146a in retinal pigment epithelial cells aging and age-related macular degeneration
郝义 1孙笑 1刘玉秋 1赵允 2王禹1
作者信息
- 1. 113006辽宁省抚顺市,抚顺市眼病医院
- 2. 200031上海市,中国科学院上海生命科学研究院
- 折叠
摘要
Abstract
Objective To investigate the expression level of miRNA-146a (miR-146a) in retinal pigment epithelial (RPE) cells aging and age-related macular degeneration (AMD),and discuss its regulation mechanism of AMD by repressing VEGF-A.Methods The expressions of miR-146 and VEGF-A were examined by qRT-PCR in RPE cells in mice aged 2 months,8 months,12 months,18 months or 24 months,and in RPE cells from 75 years old AMD patients.The protein level of VEGF-A was also detected by Western Blotting.Finally,the effects of overexpression of miR-146a in APRE-19 cell line on expression of VEGF-A was checked.Results MiR-146 was up-regulated to 8 times or 24 times at 18 months or 24 months aged mice,and the expression of VEGFA was down-regulated in aging RPE from 1.5 times to 0.8 times.However,the expression of miR-146 decreased to 14.5 times and VEGF-A increased in RPE cells of AMD.In cultured cells,overexpression of miR-146a inhibited the expression of VEGF-A.Conclusion Up-regulation of miR-146a in aging RPE cells and its down-regulation in AMD suggest a potential of miR-146a as molecular marker.MiR-146a overexpression inhibits the expression of VEGF-A,supporting a potential clinical treatment of miR-146 in AMD.关键词
miRNA-146a/视网膜色素上皮细胞/衰老/老年性黄斑变性Key words
miRNA-146 a/retinal pigment epithelial cell/aging/age-related macular degeneration分类
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郝义,孙笑,刘玉秋,赵允,王禹..miRNA-146a在视网膜色素上皮细胞老化及老年性黄斑变性中的功能研究[J].眼科新进展,2017,37(2):117-121,5.
