眼科新进展2017，Vol.37Issue(2)：121-124,4.DOI:10.13389/j.cnki.rao.2017.0032
原花青素对H2O2诱导人小梁细胞氧化应激的抗氧化作用
Protective effects of procyanidins on human trabecular meshwork cells against H2O2 induced oxidative stress
石璐 1汪昌运1
作者信息
- 1. 330006江西省南昌市,南昌大学第二附属医院眼科
- 折叠
摘要
Abstract
Objective To investigate the protection and mechanism of procyanidins (PC) against H2O2 induced oxidative damage of human trabecular meshwork cells (HTMC) in order to provide an experimental foundation for glaucoma clinical treatment.Methods HTMC were cultured and then divided randomly into 5 groups.As untreated group:Normal cultured HTMC;Control group:Normal cultured HTMC + H2O2 (500 μmol · L-1 for 1 hour);Treated group:Normal cultured HTMC + H2O2 (500 μmol ·L-1 for 1 hour) + PC (PC fmal concentrations were 0.02 g · L-1,0.05 g · L-1,0.10 g· L-1).Real-time fluorescence quantitative polymerase chain reaction (PCR) was used to investigate the expression of mitochondrial complex Ⅰ mRNA.Results Compared with untreated group (1.000 0 ± 0.000 0),the differences of mitochondrial complexⅠ mRNA expression in 0.02 g · L-1 PC (0.401 3 ±0.010 3),0.05 g · L-1 PC (0.791 5 ± 0.008 5) groups were statistically significant (all P ＜ 0.01),but the 0.10 g ·L-1 PC group (1.043 0 ± 0.062 2) had no significant differences (P ＞ 0.05).The differences between PC treated groups and control group were statistically significant (P ＜0.01),which showed HTMC treated with PC could increase the expression of mitochondrial complex Ⅰ mRNA.The differences in each PC treated groups were statistically significant (P ＜ 0.01),which showed the expression of mitochondrial complex Ⅰ mRNA were increased along with the concentration of PC gradually increased.Conclusion Exogenetic PC can increase the expression of mitochondrial complex Ⅰ mRNA in the oxidative damaged HTMC,and in a certain range of concentration,the protective effects of PC have the positive relationship of dose-effect,which suggest that PC may be a good candidate for further study of the clinical treatment of glaucoma.关键词
原花青素/小梁细胞/氧化应激/青光眼Key words
procyanidins/trabecular meshwork cells/oxidative stress/glaucoma分类
医药卫生引用本文复制引用
石璐,汪昌运..原花青素对H2O2诱导人小梁细胞氧化应激的抗氧化作用[J].眼科新进展,2017,37(2):121-124,4.
