眼科新进展2017，Vol.37Issue(2)：125-128,4.DOI:10.13389/j.cnki.rao.2017.0033
Nod样受体蛋白-3(NLRP3)炎症小体在翼状胬肉中的表达
Expression of NOD-like receptor pyrin dornain-3 (NLRP3) inflammasome in pterygium
郭切 1李志远 1荆凡波1
作者信息
- 1. 266003 山东省青岛市,青岛大学附属医院
- 折叠
摘要
Abstract
Objective To observe the expression of NOD-like receptor pyrin domain-3 (NLRP3) inflammasome and interleukin-1 beta (IL-1b) in pterygium and normal conjunctiva specimens,and clarify the role of NLRP3 in the development of pterygium.Methods Specimens from 20 cases of pterygium and 6 cases of normal eonjunctival were analyzed for establishing the expression of NLRP3 and IL-1b by immune-histochemistry.The level of caspase-1 and pro-caspase-1 protein were analyzed by Westernblot,gene expression of interleukin-18 (IL-18) was detected by RT-PCR.Results NLRP3 was not expressed in normal conjunctival epithelial cells,but was positive in the basal part of pterygium;IL-1was not expressed in normal conjunctival tissues,but was positive in pterygium.There was no significant difference about the expression of Procaspase-1 in normal conjunctiva and pterygium (P ＞ 0.05),However,caspase-1,also known as the active form of pro-caspase-1 expressed in pterygiun was higher than that in normal conjunctiva(P ＜ 0.05).The average level of IL-18 in 20 cases of pterygium was significantly higher than that in normal conjunctiva (P ＜ 0.05).Conclusion After NLRP3 signaling pathway activating in pterygium tissue,caspase-1,IL-1β and IL-18 are high expression abnormally,suggest that NLRP3 inflammasome and the related signaling pathways may play a role in the progression of pterygium.关键词
翼状胬肉/Nod样受体蛋白-3/半胱天冬酶-1/白细胞介素-1β/白细胞介素-18Key words
pterygium/NOD-like receptor pyrin domain-3/caspase-1/IL-1b/IL-18分类
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郭切,李志远,荆凡波..Nod样受体蛋白-3(NLRP3)炎症小体在翼状胬肉中的表达[J].眼科新进展,2017,37(2):125-128,4.
