眼科新进展2017，Vol.37Issue(2)：129-132,4.DOI:10.13389/j.cnki.rao.2017.0034
干眼患者眼表宏基因组研究
Study of ocular surface macro genome in dry eye patients
摘要
Abstract
Objective To investigate the difference in ocular surface microbiota between dry eye patients and healthy subjects,and discuss the role of microbiota in dry eye.Methods Twenty cases of dry eye patients and 90 cases of healthy subjects were collected in the PLA General Hospital and Zhongshan Ophthalmic Center.The samples of conjunctiva impression cytology were collected from all subjects,and then metagenomic shotgun sequencing was performed following the DNA extraction.The differences in alpha diversity and metabolic pathways of the ocular surface microbiota between dry eye patients and healthy subjects were evaluated.Results There was no significant difference in alpha diversity of the microbial community between dry eye patients and healthy subjects (P =0.13).However,an increase of 15 species and a decrease of 10 species were detected on the ocular surface of dry eye patients.The enriched antibiotic resistance genes in dry eye patients were more than healthy subjects.Conclusion Although the alpha diversity of the microbial community on ocular surface between dry eye patients and healthy subjects are not distinguishable,a significant difference could be found in relative abundance and metabolic pathways,suggest that these specific microbiome may be related to the pathogenesis and disease progression of dry eye.关键词
干眼/眼表微生物组/宏基因组测序/抗性基因Key words
dry eye/ocular surface microbiota/metagenomic shotgun sequencing/antibiotic resistance genes分类
医药卫生引用本文复制引用
陈红,文小凤,邓裕华,魏来,彭广华..干眼患者眼表宏基因组研究[J].眼科新进展,2017,37(2):129-132,4.基金项目
国家自然科学基金面上项目(编号:31271400、31470065) （编号:31271400、31470065）
国家重点基础研究发展计划(编号:2013CB967001)National Natual Science Foundation of China (No:31271400,31470065) （编号:2013CB967001）
National Key Basic Research Program of China (No:2013CB967001) （No:2013CB967001）
