中国医疗设备2017，Vol.32Issue(2)：9-15,7.DOI:10.3969/j.issn.1674-1633.2017.02.003
Leica SP8 STED 3X激光扫描共聚焦显微镜的使用研究
Research of Using Laser Scanning Confocal Microscopy Leica SP8 STED 3X
摘要
Abstract
This paper mainly introduced the components,technical parameters,working principle of confocal laser scanning microscope Leica SP8 STED 3X equipped in the Instrumental Analysis Center of Shanghai Jiao Tong University.This microscope,based on the Leica TCS SP8 confocal microscope,is equipped with super resolution model-stimulated emission depletion,live cell station and two-photon module;it can be used to carry out nano-level sub-cellular structure and dynamics study,and can also be used to observe subcellular organelle movement characteristics and changes of living cell,is a ultra-high resolution confocal microscopy system of various detecting functions.Ultra-high resolution fluorescence microscopy includes super-resolution technology based on modulation of light spot to reduce point spread function of the system,as well as super-resolution technology based on single-molecule localization.Meanwhile,preparation method of the sample,parameter setting principle of the image capture,and the routine maintenance and management,were also discussed,which has great significance in clinical practice.关键词
激光扫描/Leica SP8 STED 3X/图像采集/共聚焦/显微镜Key words
laser scanning/Leica SP8 STED 3X/image capture/confocal/microscope分类
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狄伶..Leica SP8 STED 3X激光扫描共聚焦显微镜的使用研究[J].中国医疗设备,2017,32(2):9-15,7.基金项目
科技创新专项基金(AF4040004). （AF4040004）
