中国医疗设备2017，Vol.32Issue(3)：6-9,42,5.DOI:10.3969/j.issn.1674-1633.2017.03.002
肿瘤特异性成像中固相萃取对Gd-DOTA-OCT小分子探针构建的影响
Efficacy of Brain Tumor Specific Imaging Solid Phase Extraction in the Construction of Gd-DOTA-OCT Small Molecular Imaging Probe
摘要
Abstract
Objective To discuss the effect of "one step method" on constructing Gd-Tetraazacyclododecane-Octreotide (Gd-DOTA-OCT) small molecular MR imaging probe by adopting Solid Phase Extraction (SPE)method to isolate it from the mixture. Methods The commercial DOTA-OCT was chelated with GdCl3 directly, and the mixture was isolated and purified with SPE method, then all the isolated fractions were underwent ultraviolet spectrophotometric detection, mass spectrometric detection and MR signal detection respectively. Results Gd-DOTA-OCT was synthesized with "one step method" and isolated with SPE method successfully. The actual molecular weight of the probe detected by MALTI-TOF was consistent with the theoretic molecular weight, and the Gd-DOTA-OCT MR probe was intact after being verified by the ultraviolet spectrophotometric detection and MR signal detection. Conclusion Gd-DOTA-OCT small molecular MR probe can be successfully constructed with "one step method" and can be isolated and purified with SPE method, which lays a foundation for brain tumor specific imaging.关键词
分子成像/分子探针/固相萃取/质谱检测/Gd-DOTA-OCT/MR信号检测Key words
molecular imaging/molecular probe/solid phase extraction/mass spectrometric detection/Gd-tetraazacyclododecane-octreotide/MR signal detection分类
信息技术与安全科学引用本文复制引用
霍天龙,杨硕,孙燕萍,潘峰,胡立宝,康钰,赵赟赟..肿瘤特异性成像中固相萃取对Gd-DOTA-OCT小分子探针构建的影响[J].中国医疗设备,2017,32(3):6-9,42,5.基金项目
高等学校博士学科点专项科研基金新教师类(20110001120083) （20110001120083）
国家自然科学基金面上项目(81372363). （81372363）
