针刺研究2017，Vol.42Issue(1)：14-19,6.DOI:10.13702/j.1000-0607.2017.01.003
电针“大椎”“命门”对脊髓损伤大鼠神经元细胞凋亡及JNK信号通路相关蛋白表达的影响
Effect of Electroacupuncture Stimulation at "Dazhui" (GV 14) and "Mingmen"(GV 4) on Cell Apoptosis of Spinal Cord and Expression of JNK Signaling Related Protein in Spinal Cord Injury Rats
摘要
Abstract
Objective To observe the effect of electroacupuncture (EA) stimulation at "Dazhui" (GV 14) and "Mingmen" (GV 4) of the Governor Vessel at different time-points on spinal cord neuronal apoptosis and the expression of c-Jun N-terminal kinases (JNK) protein in spinal cord injury (SCI) rats,so as to reveal its mechanism underlying improving SCI.Methods A total of 108 male SD rats were randomly divided into normal control,SCI model and EA groups which were further divided into 1,3 and 7 d subgroups (12 rats/subgroup,6 rats in each subgroup for TUNEL or Western blot,separately).SCI model was established by using the modified Allen's method.EA was applied to GV 14 and GV 4 for 20 min,once daily,for 1,3 and 7 days,respectively.Basso-Beattie-Bresnahan (BBB) scale was adopted to assess the locomotor function of rats,the TUNEL method was used to examine neuronal apoptosis of injuried spinal cord,and the expression of phosphorylated (p)-c-Jun protein of T9-T11 spinal cord was detected by using Western blot.Results After modeling,the BBB scores of SCI rats on day 1,3 and 7 were significantly decreased (P＜0.01),while the numbers of apoptotic neuronal cells and the expression levels of p-c-Jun protein in the spinal cord were considerably increased at the 3 time-points in the model group (P＜0.01,P＜0.05).Following EA intervention,the decreased BBB scores on day 3 and 7,and the increased numbers of apoptotic neuronal cells on day 1,3 and 7 and the upregulated expression levels of p-c-Jun protein on day 3 and 7 were obviously suppressed (P＜0.05,P＜0.01).Conclusion EA intervention can improve the locomotor function of SCI rats,which may be related to its effects in reducing neuronal apoptosis and down-regulating p-c-Jun protein in the injuried spinal cord.关键词
脊髓损伤/电针/JNK信号通路/细胞凋亡Key words
Spinal cord injury/Electroacupuncture/c-Jun N-terminal kinases (JNK)/Neuronal apoptosis分类
医药卫生引用本文复制引用
吕威,时素华,李冰,景泉凯,莫雨平,姚海江,宋良玉,王鑫,毛颖秋,李志刚..电针“大椎”“命门”对脊髓损伤大鼠神经元细胞凋亡及JNK信号通路相关蛋白表达的影响[J].针刺研究,2017,42(1):14-19,6.基金项目
国家自然科学基金面上项目(No.81373728) （No.81373728）
