针刺研究2017，Vol.42Issue(1)：20-24,49,6.DOI:10.13702/j.1000-0607.2017.01.004
双强度、频度电针对血管性痴呆大鼠海马CA1区β淀粉样蛋白1-40、精氨酸加压素及学习记忆的影响
Influence of Electroacupuncture Stimulation with Different Intensities and Therapeutic Intervals on Learning-memory Ability and Expression of Aβ 1-40 and Arginine Vasopressin Genes in the Hippocampal CA 1 Region in VD Rats
摘要
Abstract
Objective To observe the influence of electroacupuncture (EA) stimulation with different electrical current intensities and therapeutic intervals on learning-memory ability and expression of β-amyloid peptide Aβ 1-40 and arginine vasopressin (AVP) genes in the hippocampal CA 1 region in vascular dementia (VD) rats,so as to provide evidence for treatment of VD.Methods A total of 48 male SD rats were randomly divided into sham,model,0.5 mA-5 d-EA,1.5 mA-5 d-EA,0.5 mA-1 d-EA and 1.5 mA-1 d-EA groups (n =8 in each group).The VD model was established using modified 4-vessels occlusion method.EA (alternative 2 Hz/15 Hz,0.5 mA,1.5 mA) was applied to "Baihui" (GV 20) and "Dazhui" (GV 14) for 30 min,once a day (1 d) or once every 5 d for 10 times.The learning-memory ability was detected using Morris water maze tests (place navigation task and spatial probe trials),and the expression of Aβ 1-40 and AVP genes in hippocampal CA 1 region was determined using real time-PCR.Results In comparison with the sham group,the average escape latency of place navigation task,and the duration for crossing the target-platform for the 1st time (spatial navigation task) were significantly increased (P＜0.05),and the times to cross the target-platform within 2 min (spatial probe trials) were significantly decreased in the model group (P＜0.05),suggesting a reduction of learning-memory ability.The expression level of Aβ 1-40 mRNA was remarkably increased (P＜0.05),and that of AVP mRNA notably decreased in the model group (P＜0.05).Following EA intervention,the increased escape latency and the duration of crossing the target-platform for the 1st time and the increased Aβ 1-40 mRNA expression,and the decreased target-platform crossing times within 2 min as well the down-regulated AVP mRNA expression level were reversed in the 4 EA groups compared with the model group (P＜0.05).The therapeutic effects of higher stimulating intensity (1.5 mA-1 d-EA and 1.5 mA-5 d-EA) were markedly superior to those of lower intensity (0.5 mA-1 d-EA and 0.5 mA-5 d-EA),and the effects of higher frequency of EA intervention (0.5 mA-5 d-EA and 1.5 mA-5 d-EA) were obviously superior to those of lower frequency of EA (0.5 mA-1 d-EA and 1.5 mA-1 d-EA) in suppressing the increased 3 indexes and the decreased 2 indexes mentioned above (P＜0.05).Conclusion EA can improve the learning-memory ability of VD rats,which may be related to its effects in inhibiting the expression of Aβ 1-40 mRNA and up-regulating the expression of AVP mRNA in hippocampal CA 1 region;and the therapeutic effects of higher stimulating intensity and higher intervention frequency are obviously better than those of lower stimulating intensity and lower therapeutic frequency.关键词
血管性痴呆/电针/电针强度/频度/β淀粉样蛋白1-40/精氨酸加压素/学习记忆能力Key words
Vascular dementia/Electroacupuncture/Stimulating intensity/therapeutic interval/Aβ 1-40/Arginine vasopressin/Learning-memory ability分类
医药卫生引用本文复制引用
蒋灵鸽,张华伟,张拯,邵瑛..双强度、频度电针对血管性痴呆大鼠海马CA1区β淀粉样蛋白1-40、精氨酸加压素及学习记忆的影响[J].针刺研究,2017,42(1):20-24,49,6.基金项目
中国博士后科学基金第55批面上资助项目(No.2014 M 550997) （No.2014 M 550997）
