针刺研究2017，Vol.42Issue(1)：25-30,6.DOI:10.13702/j.1000-0607.2017.01.005
电针“夹脊”穴预处理对心肌缺血再灌注大鼠细胞色素P450信号通路基因表达的影响
Effect of "Jiaji" (EX-B 2)-electroacupuncture Preconditioning on Expression of Cytochrome P 450 Signaling Pathways in Myocardial Ischemia-reperfusion Rats
摘要
Abstract
Objective To investigate the effect of "Jiaji"(EX-B 2)-electroacupuncture(EA) preconditioning on the myocardium and expression of cytochrome P 450 signaling pathways of myocardial cells in myocardial ischemia-reperfusion injury(MI/RI) rats.Methods Sixty male Wistar rats were randomly divided into sham operation,model,Jiaji,Neiguan(PC 6),Yanglingquan(GB 34) and Quchi(LI 11) groups (n =10 in each group).MI/RI model was established by occlusion of the descending anterior branch of the left coronary artery.EA preconditioning was respectively applied to EX-B 2,PC 6,GB 34 and LI 11 for 30 min,once daily for 7 days.The ECG-ST segment was detected before and after the modeling.The contents of serum creatine kinase-MB(CK-MB) and lactate dehydrogenase(LDH) were detected by ELISA and microplate method separately.The expression levels of myocardial CYP 450 related genes cyp 1 a 1,cyp 2 b 2,cyp 2 c 11,cyp 4 a 2 were detected by Real-time PCR.Results Thirty minutes after ligation the changes of ECG-ST segment displacement of the Jiaji group and Neiguan group were significantly lower than that of the model group(P＜0.01),and the change was significantly lower in the Neiguan group than in the model group(P＜0.01) 30 minutes after reperfusion.Compared with the sham operation group,the activities of serum CK-MB and LDH were significantly increased in the model group(P＜0.01).Compared with the model group,the activities of serum CK-MB and LDH were significantly decreased in the Jiaji,Neiguan and Yanglingquan groups (P＜0.01).Compared with the Jiaji group,the activity of serum LDH was significantly increased in the Quchi group,while the activities of serum CK-MB were significantly increased in the Quchi and Yanglingquan groups (P＜0.05).Compared with the sham operation group,the expression levels of myocardial cyp 1 a 1 and cyp 2 b 2 mRNAs were significantly higher in the model group(P＜0.01),while the expression of cyp 2 c 11 mRNA was lower(P＜0.01).Compared with the model group,the expression levels of cyp 1 a 1 and cyp 4 a 2 mRNAs were lower in the Jiaji,Neiguan and Yanglingquan groups (P＜0.01＜,while the expressions of cyp 2 b 2 and cyp 2 c 11 mRNAs were higher（P＜0.01）.Compared with the Jiaji group,the expression of cyp 1 a 1 mRNA was lower in the Neiguan group,while the expression of cyp 2 b 2 mRNA was higher(P＜0.05).Conclusion EA preconditioning at Jiaji(EX-B 2)can reduce serum myocardial enzymes of myocardial ischemia-reperfusion rats,lower the ST segment of electrocardiogram,up-regulate myocardial CYP 450 epoxygenase cyp 2 b 2 and cyp 2 c 11 mRNAs expression,down-regulate ω-hydroxylase cyp 1 a 1 and cyp 4 a 2 mRNAs expression,thus play a role in myocardial protection.关键词
电针/预处理/心肌缺血再灌注/心肌酶/细胞色素P 450Key words
Electroacupuncture/Preconditioning/Myocardial ischemia-reperfusion/Myocardial enzyme/Cytochrome P 450分类
医药卫生引用本文复制引用
李洋,邵明璐,于慧娟,谭奇纹,崔华峰..电针“夹脊”穴预处理对心肌缺血再灌注大鼠细胞色素P450信号通路基因表达的影响[J].针刺研究,2017,42(1):25-30,6.基金项目
国家自然科学基金项目(No.81373722) （No.81373722）
山东省自然基金联合专项(No.ZR 2014 HL 100) （No.ZR 2014 HL 100）
