针刺研究2017，Vol.42Issue(1)：31-38,8.DOI:10.13702/j.1000-0607.2017.01.006
针灸预处理对心肌缺血再灌注损伤兔不同时间热休克蛋白27、70、90表达的影响
Effects of Electroacupuncture and Moxibustion Pretreatment on Expressions of HSP 27, HSP 70, HSP 90 at Different Time-points in Rabbits with Myocardial Ischemia-reperfusion Injury
摘要
Abstract
Objective To observe the effect of electroacupuncture (EA) and moxibustion (Moxi) pretreatment on expression of myocardial heat shock protein (HSP) in acute myocardial ischemia-reperfusion injury (MIRI) rabbits.Methods A total of 72 New Zealand rabbits were randomly divided into 4 groups:sham operation,MIRI model,EA pretreatment and Moxi pretreatment (n =18 rabbits in each group) which were further divided into 0,24 and 48 h (time-point) subgroups (n =6 in each).The MIRI model was established by occlusion of the anterior descending branch (ADB) of the left coronary artery for 40 min and reperfusion for 60 min.EA and Moxi stimulation was respectively applied to bilateral "Neiguan"(PC,6) for 20 min,once daily for 5 days before ADB occlusion.The expressions of myocardial HSP 27,HSP 70 and HSP 90 were detected by immunohistochemistry.The pathological and ultrastructural changes of left ventricular ischemia tissue were observed under light and transmission electronic microscope (TEM),respectively.Results Outcomes of H.E.staining and ultrastructure showed that MIRI-induced changes of disordered arrangerrnt of cardiomyocytes,vague myocardial transverse striation,inflammatory infiltration,cardiac myofibre necrosis and fibrolysis (light microscope),and myofiber atrophy,vague and disorder in the arrangement of myofiber,myofilament necrosis,interstitial edema,mitochondrial swelling,microvessel expansion,etc.(TEM) were relatively milder in both EA and Moxi pretreatment groups (48 h).In comparison with the sham group,the expression levels of myocardial HSP 27,HSP 70 and HSP 90 had no significant changes after MIRI at the 3 time-points (P＞0.05).In the pretreatment groups,the expression levels of HSP 27 at 24 and 48 h in both EA and Moxi groups,HSP 70 at 48 h in both groups,HSP 70 at 0 and 24 h in the Moxi group were significantly up-regulated compared with the model group (P＜0.05,P＜0.01).No significant changes were found in the expression of HSP 90 at the 3 time-points in the EA and Moxi pretreatment groups (P＞0.05).No significant differences were found between EA and Moxi in up-regulating expressions of myocardial HSP 27,HSP 70 and HSP 90 proteins at the 3 time-points (P＞0.05) except HSP 70 at 24 h (Moxi being stronger relative to EA,P＜0.05).Conclusion EA and Moxi pretreatment has a protective effect on ischemic myocardium in MIRI rabbits,which may be associated with their actions in up-regulating myocardial HSP 27 and HSP 70 expression.关键词
针灸预处理/心肌缺血再灌注/热休克蛋白27/热休克蛋白70/热休克蛋白90Key words
Electroacupuncture pretreatment/Moxibustion pretreatment/Myocardial ischemia-reperfusion/HSP 27/HSP 70/HSP 90分类
医药卫生引用本文复制引用
谭成富,严洁,王超,常小荣,谢文娟,阳晶晶,刘密,林海波,贺香嫦..针灸预处理对心肌缺血再灌注损伤兔不同时间热休克蛋白27、70、90表达的影响[J].针刺研究,2017,42(1):31-38,8.基金项目
国家自然科学基金面上项目(No.81072868) （No.81072868）
国家自然科学基金青年基金项目(No.81102661) （No.81102661）
湖南省中医药管理局课题(No.201521) （No.201521）
湖南省高校创新平台开放基金项目(No.15 k 093) （No.15 k 093）
