针刺研究2017，Vol.42Issue(1)：39-44,6.DOI:10.13702/j.1000-0607.2017.01.007
电针对高脂饮食诱导的肥胖小鼠白色脂肪组织中腺苷受体表达的影响
Effect of Electroacupuncture on Adenosine Receptor Expression in White Adipose Tissue of Dietinduced Obese Mice
摘要
Abstract
Objective To investigate the effects of electroacupuncture (EA) on the expression of adenosine receptor (AR) in the white adipose tissue (WAT) of diet-induced obese (DIO) mice,so as to reveal a peripheral mechanism of EA underlying improvement of body weight.Methods Forty three-week-old C 57 BL/6 male mice were divided into normal diet group (n =12) and high fat diet group (n =28) randomly,and fed by normal diet and high fat diet for 12 weeks,respectively.In the high fat diet group,mice with body weight over 20％ heavier than that of the normal diet group were considered as obese mice.The normal diet mice and the obese mice were divided into normal group (CD,n =5) and normal plus EA group (CD + EA,n =7),or obese group (HFD,n =6) and obese plus EA group (HFD + EA,n =12).The CD 4+EA group and the HFD + EA group were treated with EA at "Zusanli"(ST 36) and "Neiting"(ST 44,2 Hz/15 Hz,0.6-1.0 mA) for 20 min,6 times a week for 4 weeks.Body weight,ratio of WAT/body weight were calculated,qPCR and Western blot were applied to detect mRNA and protein levels of adenosine receptors in the epididymal adipose tissue (Epi-WAT),respectively.Results Compared with the normal diet group,high fat diet significantly increased body weight in C 57 BL/6 mice after feeding for 12 weeks (P＜0.01);18 out of 28 mice in the high fat diet group were classified as obesity.After treatment,the body weight and the ratio of Epi-WAT/body weight of the HFD group were increased than those in the CD group (P＜0.05),the change of body weight in the HFD group was bigger than that in the CD group (P＜0.01).Compared with the HFD group,the body weight and the ratio of Epi-WAT/body weight of the HFD + EA group were decreased after EA (P＜0.05),the change of body weight was also significantly increased (P＜0.01).No significant differences were found among the four groups in the expression level of A1R mRNA (P＞0.05).The expression of A3 R mRNA in the HFD group was lower than that in the CD group (P＜0.01),while the expressions of A2A R and A2B R proteins were decreased in the HFD group than in the CD group (P＜0.01).In comparison with the HFD group,the expression levels of A2AR and A2BR mRNAs and proteins were significantly up-regulated in the HFD + EA group,respectively (P＜0.05,P＜0.01).Conclusion EA intervention is able to reduce the body weight of DIO mice,which may be associated with its effects in regulating the expression of A2AR and A2BR in WAT,suggesting a new mechanism of EA in accelerating peripheral WAT metabolism.关键词
电针/肥胖/白色脂肪组织/脂肪代谢/腺苷受体Key words
Electroacupuncture/Obesity/White adipose tissue/Lipocyte metabolism/Adenosine receptor分类
医药卫生引用本文复制引用
汤月霞,孟建忠,丁亚娟,洪浩,于美玲,景欣悦,卢圣锋,朱冰梅..电针对高脂饮食诱导的肥胖小鼠白色脂肪组织中腺苷受体表达的影响[J].针刺研究,2017,42(1):39-44,6.基金项目
国家自然科学基金(No.81273838、81303019、81303018) （No.81273838、81303019、81303018）
