诊断学理论与实践2017，Vol.16Issue(1)：93-97,5.DOI:10.16150/j.1671-2870.2017.01.018
尿液多种microRNA检测方法的建立及其在膀胱癌诊断中的应用研究
Establishment of urinary micro-RNA detection method and its application in diagnosis of bladder cancer
李莉 1卞炳贤 1张良 2沈立松1
作者信息
- 1. 上海交通大学医学院附属新华医院检验科,上海 200092
- 2. 上海交通大学医学院附属新华医院泌尿外科,上海 200092
- 折叠
摘要
Abstract
Objective:To establish a method for detection of 8 urinary micro-RNA and to detect their distribution in urine and evaluating its significance in detection of bladder cancer. Methods: Early morning and random urine samples from 15 healthy controls and random urine samples from 15 patients with bladder cancer were collected. The total RNA were extracted and then were reversely transcripted to cDNA, and 8 micro-RNAs (miR-126, miR-200a, miR-200b, miR-200c, miR-182, miR-183, miR-429 and miR-141) were amplified with real-time polymerase chain reaction (PCR). Diffe-rences in miRNA levels were compared between early morning and random urine samples as well as between non-concen-trated and concentrated samples. Different extraction and purification methods for miRNAs were compared and the diffe-rences of miRNA concentrations between controls and bladder cancer patients were analyzed. The diagnostic efficacy of multiple urinary miRNA combinations for bladder cancer was analyzed using ROC curve. Results: The relative concentra-tions of 6 miRNAs (miR-126, miR-200a, miR-200b, miR-200c, miR-429 and miR-141) were significantly higher in the concentrated urine than those in non-concentrated urine. There was no significant difference in relative miRNA concentra-tion between early morning and randomized urine collection. Compared with normal controls, the relative concentrations of 4 miRNAs (miR-126, miR-182, miR-183 and miR-141) were significantly increased in bladder cancer group while miR-NA-200a was significantly decreased. ROC analysis showed that detection of single miRNA had limited diagnostic efficacy for bladder cancer, while combined analysis of 5 miRNA had better diagnostic value for bladder cancer. Conclusions:Multiple micro-RNAs are stably distributed in urine, and combined detection of multiple miRNAs might be used as a dia-gnostic marker for bladder cancer.关键词
膀胱癌/微小RNA/尿液/实时聚合酶链反应Key words
Bladder cancer/MicroRNA/Urine/Real-time polymerase chain reaction分类
医药卫生引用本文复制引用
李莉,卞炳贤,张良,沈立松..尿液多种microRNA检测方法的建立及其在膀胱癌诊断中的应用研究[J].诊断学理论与实践,2017,16(1):93-97,5.
