中风与神经疾病杂志2017，Vol.34Issue(2)：137-140,4.
SH-SY5Y细胞α7尼古丁受体蛋白抑制对tau蛋白及p38MAPK通路的影响及其与AD发病机制的关系
Influence of inhibited α7 nicotinic acetylcholine receptor protein expression on tau protein and p38 MAPK signal transduction pathway in SH-SY5Y cells and the relationship with the pathogenesis of AD
摘要
Abstract
Objective To investigate the influence of inhibited protein expression of α7 neuronal nicotinic acetyl-choline receptor ( nAChR) on tau phosphorylation and the relationship with p 38 MAPK signal transduction pathway in SH-SY5Y cells,to study the effected mechanism of α7 nAChR on tau phosphorylation .Methods SH-SY5Y cells were exposed to MLA and SB203580,respectively,to block the activation and protein expression of α7 nAChR and p38 MAPK pathway. The protein levels of tau,p-tau (S404),p-tau (S214),α7 nAChR,p38 MAPK and p-p38 MAPK (Thr180/Tyr182) were measured by Western blotting .Results As compared with negative controls ,in MLA treated group ,the expressions of p-tau ( S404 ) and p-tau ( S214 ) were increased and the protein levels of α7 nAChR and p-p38 MAPK were decreased signifi-cantly,tau and p38 MAPK protein expressions were unchanged .In SB203580 treated group and both two medicines treated group,the expressions of p-tau (S404),p-tau (S214),α7 nAChR and p-p38 MAPK were both decreased conspicuously ,the protein levels of tau and p38 MAPK were still unchanged.Conclusions Blocked p38 MAPK signal transduction pathway can prevent against the α7 nAChR-induced tau hyperphosphorylation .关键词
阿尔茨海默病/tau/α7nAChR/p38MAPKKey words
Alzheimer ' s disease/tau/Receptors/Cholinergic/p38 MAPK分类
医药卫生引用本文复制引用
周飞,李毅,吴昌学,官志忠,齐晓岚..SH-SY5Y细胞α7尼古丁受体蛋白抑制对tau蛋白及p38MAPK通路的影响及其与AD发病机制的关系[J].中风与神经疾病杂志,2017,34(2):137-140,4.基金项目
国家自然科学基金资助项目(81360178) （81360178）
教育部"长江学者和创新团队发展计划资助"(IRT13058) （IRT13058）
贵州省科技厅重大专项[黔科合重大专项字2014(6008)] （6008）
贵州省科技厅项目[201344,黔科合SY字(2013)][3020黔科合LH字(2016)7365] （2013）
