中国病理生理杂志2017，Vol.33Issue(3)：411-416,6.DOI:10.3969/j.issn.1000-4718.2017.03.005
黄芪甲苷对慢性心衰大鼠心肌纤维化及能量代谢的影响
Effects of astragaloside IV on myocardial fibrosis and energy metabolism in chronic heart failure rats
摘要
Abstract
AIM:To observe the effects of astragaloside IV (AS-IV) on myocardial fibrosis in chronic heart failure ( CHF) rats and to explore the underlying mechanism preliminarily .METHODS:Chronic heart failure model rats established by abdominal aorta constriction (AAC) were divided into CHF group, valsartan group and AS-IV group.Sham operation group was also established .The rats in valsartan group and AS-IV group received valsartan and AS-IV at 2 and 30 mg· kg-1 · d-1 , respectively.The rats in sham operation group and CHF group received normal saline .After 8 weeks of treatment, the cardiac structure and the hemodynamic parameters were measured .The morphologic changes of myocardial tissue were observed after staining .The expression of long-chain acyl-CoA dehydrogenase ( LCAD) and 6-phosphofructoki-nase-1 (PFK1) at mRNA and protein levels was determined by RT-qPCR and Western blot.RESULTS:Compared with sham operation group , left ventricular mass index ( LVMI) , collagen volume fraction ( CVF) , left ventricular posterior wall depth (LVPWD), and the mRNA and protein of PFK1 in CHF group were increased (P<0.05), while the mRNA and protein levels of LCAD were decreased (P<0.05).Compared with CHF group, the LVMI, CVF, LVPWD, and the mRNA and protein levels of PFK1 in valsartan group and AS-IV group were decreased (P<0.05), while the mRNA and protein levels of LCAD were increased (P<0.05).CONCLUSION:AS-IV inhibits myocardial fibrosis in the CHF rats , the mechanism of which might be associated with up-regulating the expression of LCAD , down-regulating the expression of PFK1 and normalizing the myocardial energy metabolism .关键词
能量代谢/黄芪甲苷/慢性心力衰竭/心肌纤维化Key words
Energy metabolism/Astragaloside IV/Chronic heart failure/Myocardial fibrosis分类
医药卫生引用本文复制引用
唐斌,张金国,谭洪勇,尉希清..黄芪甲苷对慢性心衰大鼠心肌纤维化及能量代谢的影响[J].中国病理生理杂志,2017,33(3):411-416,6.基金项目
山东省高校优秀科研创新团队计划资助项目(鲁教科字[2012]8号) （鲁教科字[2012]8号）
济宁医学院重点科研计划(No.JY2013KJ015) （No.JY2013KJ015）
