中国病理生理杂志2017，Vol.33Issue(3)：505-509,5.DOI:10.3969/j.issn.1000-4718.2017.03.020
钙敏感受体通过PI3K/AKT通路对缺氧诱导的A549细胞增殖的影响
Calcium-sensing receptor mediates hypoxia-induced proliferation of A549 cells through PI3K/AKT pathway
摘要
Abstract
AIM:To explore the cell signal transduction pathway of calcium-sensing receptor ( CaSR) media-ting hypoxia-induced proliferation of A549 cells and A549/DDP cells.METHODS: The cell proliferation was tested by BrdU incorporation assay .The cell cycle analysis was carried out by flow cytometry .The protein levels of PCNA and p-AKT were analyzed by Western blot .RESULTS: Hypoxia significantly increased the protein levels of PCNA and p-AKT, the BrdU incorporation and the cell proliferation index .GdCl3 ( an agonist of CaSR ) amplified the effect of hypoxia .LY294002 (a PI3K inhibitor) decreased the up-regulation of PCNA expression, the BrdU incorporation and the increased cell prolifer-ation index induced by hypoxia and GdCl 3 in the A549 cells and A549/DDP cells.CONCLUSION:CaSR is expressed in A549 cells and 549/DDP cells, and CaSR activation induced by hypoxia promotes the proliferation of A 549 cells and A549/DDP cells through PI3K/AKT signaling pathway .关键词
钙敏感受体/A549细胞/缺氧/细胞增殖/PI3K/AKT信号通路Key words
Calcium-sensing receptor/A549 cells/Hypoxia/Cell proliferation/PI3K/AKT signaling pathway分类
医药卫生引用本文复制引用
李光伟,苗宏志,李波,沈云虹,邱丽萍,张亚珍,金香兰,朱坤杰..钙敏感受体通过PI3K/AKT通路对缺氧诱导的A549细胞增殖的影响[J].中国病理生理杂志,2017,33(3):505-509,5.基金项目
齐齐哈尔医学院博士基金资助项目(No.QY2016B-16) （No.QY2016B-16）
