中国病理生理杂志2017，Vol.33Issue(3)：528-533,6.DOI:10.3969/j.issn.1000-4718.2017.03.024
甘草酸对博莱霉素诱导的实验性肺纤维化的干预作用
Effect of glycyrrhizic acid on bleomycin-induced pulmonary fibrosis
摘要
Abstract
AIM:To investigate the influence of glycyrrhizic acid ( GA) on bleomycin ( BLM)-induced pulmo-nary fibrosis.METHODS:C57BL/6 male mice were randomly divided into normal saline (NS) group, BLM group, BLM+NS group and BLM +GA group.The mice were administered with BLM (2.5 mg/kg) via oropharyngeal aspiration .GA (40 mg/kg) or NS was delivered daily via oral gavage after BLM administration .The mice were sacrificed on days 3, 7, 14 and 21.HE staining and Masson's trichrome staining were used to conduct histopathologic examination .The proportions of different circulatory monocytes subsets and polarization state of the bronchoalveolar lavage fluid ( BALF) -derived alveolar macrophages (AM) were measured by flow cytometry.The mRNA expression of transforming growth factor-β1 (TGF-β1) was examined by RT-qPCR.RESULTS: Compared with BLM +NS group, GA significantly alleviated BLM-induced in-flammation and collagen levels , and then decreased the proportions of Ly 6Chi monocytes on day 3, 7 and AM M2 on day 7, 14.GA effectively down-regulated the mRNA level of TGF-β1 and the content of HYP in the BLM-treated lung tissues .CONCLUSION:GA attenuates the BLM-induced pulmonary inflammation and fibrosis , which may be related to adjusting and controlling a dynamic view of mononuclear phagocyte changes and down -regulation of TGF-β1 in the lung tissues .关键词
甘草酸/肺纤维化/单核细胞/肺泡巨噬细胞/转化生长因子β1Key words
Glycyrrhizic acid/Pulmonary fibrosis/Monocytes/Alveolar macrophage/Transforming growth factor-β1分类
医药卫生引用本文复制引用
李祎,李鑫,李琦,苏程程,周欣,彭守春,魏路清,姬文婕..甘草酸对博莱霉素诱导的实验性肺纤维化的干预作用[J].中国病理生理杂志,2017,33(3):528-533,6.基金项目
国家自然科学基金资助项目(No.81102088 （）
No.81441101 （）
No.81570335) （）
天津市自然科学基金资助项目(No. 15JCZDJC35000) （No. 15JCZDJC35000）
武警后勤学院附属医院重点项目(No.FYZ201510 （）
No.FYZ201605) （）
