中国耳鼻咽喉颅底外科杂志2017，Vol.24Issue(1)：20-23,4.DOI:10.16066/j.1672-7002.2017.01.003
血清微小RNA-222-3p在甲状腺乳头状癌中的表达及意义
Clinical significance of serum microRNA-222-3p expression in papillary thyroid carcinoma
摘要
Abstract
OBJECTIVE To investigate the expression of serum miR-222-3p in papillary thyroid carcinoma and its clinical significance.METHODS Total RNA in serum was extracted from 121 patients of papillary thyroid carcinoma and benign thyroid diseases. The reverse transcription quantitative real-time polymerase chain reaction(qRT-PCR) method was used to detect the expression of miR-222-3p, and then the potential correlation between serum miR-222-3p and clinical pathological characteristics of papillary thyroid carcinoma was analyzed. The receiver operating characteristic area under the curve(ROC-AUC) and their index for diagnosis evaluation were also calculated.RESULTS The median expression level of serum miR-222-3p in papillary thyroid carcinoma patients was significantly higher than that of control group (2.2188 vs 0.7022,P=0.002). Although the expressions of serum miR-222-3p was not associated with the gender, age, capsule invasion, TNM stage, but it was positively correlated with tumor size,bilateral involvement, lesion quantity and lymph node status. ROC curve analysis showed that the specificity and sensitivity of the miR-222-3p diagnosis of papillary thyroid cancer were 79.75% and 61.90%, respectively, while the ROC-AUC was 0.717.CONCLUSION The serum miR-222-3p is over expressed in papillary thyroid carcinoma and its expression is significantly correlated with tumor progression. It will be helpful for PTC diagnosis.关键词
甲状腺肿瘤/微RNAs/诊断/微小RNA-222-3p/侵袭性Key words
Thyroid Neoplasms/MicroRNAs/Diagnosis/microRNA-222-3p/aggressiveness引用本文复制引用
丁金旺,叶柳青,王克义,罗定存,屠巧峰,张煜,时晶晶,彭友,张卧,潘钢..血清微小RNA-222-3p在甲状腺乳头状癌中的表达及意义[J].中国耳鼻咽喉颅底外科杂志,2017,24(1):20-23,4.基金项目
浙江省公益技术应用研究项目(2017C33180)、浙江省医药卫生科技计划项目(2014KYB203)、杭州市重大科技创新专项项目(20131813A08)、杭州市医药卫生科技计划项目(2012B004)、杭州市科技计划项目(20160533B05)和2016年杭州市科技计划项目(20160533B69)联合资助 （2017C33180）
