中国康复理论与实践2017，Vol.23Issue(3)：257-262,6.DOI:10.3969/j.issn.1006-9771.2017.03.003
盐酸法舒地尔对蛛网膜下腔出血大鼠学习记忆功能及海马CA1区自噬的影响
Effects of Fasudil Hydrochloride on Learning and Memory Ability and Autophagy in CA1 Area of Hippocampus in Subarachnoid Hemorrhage Rats
摘要
Abstract
Objective To investigate the effects of fasudil hydrochloride on learning and memory, and the autophagy in hippocampal CA1 neurons in subarachnoid hemorrhage (SAH) rats. Methods Fifty-four male Sprague-Dawley rats were randomly divided into sham group (n=18), SAH group (n=18) and drug group (n=18). Subarachnoid hemorrhage model was established with internal carotid artery punc-ture. The drug group was injected fasudil hydrochloride 10 mg/kg intraperitoneally after modeling per 24 hours, while the sham group and SAH group were injected the same volume of saline. They were tested with shuttle box test 6, 24 and 72 hours after intervention, then the hippocampal CA1 area was stained with HE and immunohistochemistry to observe the morphology of cells and the expression of Beclin-1 and microtubule-associated protein 1 light chain 3 II (LC3-II). Results Compared with the sham group, the frequence of avoidance de-creased in SAH group at each time point (P<0.05), while the avoidance reaction time increased (P<0.05);the survival of neurons in hippo-campal CA1 area decreased (P<0.05), and the expression of Beclin-1 and LC3-II increased (P<0.05). Compared with SAH group, the fre-quence of avoidance increased in the drug group at each time point (P<0.05), while the avoidance reaction time decreased (P<0.05);the sur-vival of neurons in hippocampal CA1 area increased (P<0.05) and the expression of Beclin-1 and LC3-II increased further (P<0.05). Con-clusion Fasudil hydrochloride can improve learning and memory ability and protect neurons from damage, which may associate with the ex-cess of autophagy activation in hippocampal CA1 areas in SAH rats.关键词
蛛网膜下腔出血/盐酸法舒地尔/学习记忆/自噬/大鼠Key words
subarachnoid hemorrhage/fasudil hydrochloride/learning and memory/autophagy/rats分类
医药卫生引用本文复制引用
孙林林,刘振刚,张志勇,陈扬,刘俊杰,付爱军,李建民..盐酸法舒地尔对蛛网膜下腔出血大鼠学习记忆功能及海马CA1区自噬的影响[J].中国康复理论与实践,2017,23(3):257-262,6.基金项目
河北省重大医学课题(No.ZD2013093). （No.ZD2013093）
