中国康复理论与实践2017，Vol.23Issue(3)：298-303,6.DOI:10.3969/j.issn.1006-9771.2017.03.010
次声对大鼠海马区钙调依赖性蛋白激酶Ⅱ及Tau蛋白表达的影响
Effects of Infrasound on Expression of Calmodulin-dependent Protein Kinase II and Tau Protein in Hippocam-pus of Rats
摘要
Abstract
Objective To study the effect of infrasound on expression of calmodulin-dependent protein kinase II (CaMKII) and tau pro-tein in hippocampus of rats. Methods Fifty-six male Sprague-Dawley rats were randomized into control group (n=8), 1-day group (n=8), 7-day group (n=8) and 14-day group (n=32), and the 14-day group was subgrouped as 1-hour, 6-hour, 24-hour, 48-hour subgroups, naming after the time after infrasound exposure, 8 in each subgroup. All the test groups were put in an infrasound field with 8 Hz, 130 dB for 2 hours daily, while the control group was put in the infrasound instrument without infrasound exposure for 2 hours daily. The expression of pT286-CaMKII and tau protein in hippocampus was detected with immunohistochemisty, Western blotting and enzyme-linked immunoabsor-bent assay. Results The expression of pT286-CaMKII was the most in 14-day group (F>14.912, P<0.001), as well as the expression of tau pro-tein (F>36.229, P<0.001), and secondary in 7-day group (P<0.05). For 14-day group, the expression of tau protein was the most in 1-hour and 6-hour subgroups, and dropped down in 24-hour subgroup, although more than that in the control group (P<0.05). Conclusion Exposure of 8 Hz, 130 dB infrasound may induce phosphorylation of CaMKII and tau protein, and the expression of tau protein in hippocampal cells in rat, which may disturb their learning and memory function.关键词
次声/钙调依赖性蛋白激酶Ⅱ/tau蛋白/学习记忆/海马/大鼠Key words
infrasound/calmodulin-dependent protein kinase II/tau protein/learning and memory/hippocampus/rats分类
医药卫生引用本文复制引用
蔡丽,王晋,崔尧,张鸽,武松艳,张文东,刘朝晖..次声对大鼠海马区钙调依赖性蛋白激酶Ⅱ及Tau蛋白表达的影响[J].中国康复理论与实践,2017,23(3):298-303,6.基金项目
国家自然科学基金项目(No.31470825). （No.31470825）
