中国临床药理学与治疗学2017，Vol.22Issue(2)：124-131,8.
烟碱通过N1受体促进热应激对RAW264.7细胞HSP70的诱导表达
Nicotine enhanced the inducible expression of HSP70 under heat stress through N1 receptor in RAW264.7 macrophages
谌崇峰 1杨怀才 2涂自智3
作者信息
- 1. 暨南大学附属第一医院儿科,广州510632,广东
- 2. 中南大学湘雅医学院病理学与病理生理学教研室,长沙410078,湖南
- 3. 清远市妇幼保健院外科,清远511500,广东
- 折叠
摘要
Abstract
AIM:To explore whether low doses of nicotine enhances the HSF1 nuclear translocation and expression of HSP70 in the RAW264.7 macrophages treated under lower heat stress (＜ 40 ℃),and to uncover its receptor mechanism.METHODS:After stimulating with nicotine at doses of 1 mmol/L and its antagonists prior to nicotine treatment at 37 ℃ or 39 ℃,the expression of HSP70 in RAW264.7 macrophages was detected by Westernblot,translocation of HSF1 was detected by immunofluorescence and the combination of HSF1 and heat shock element (HSE) was detected by EMSA,respectively.RESULTS:Western-blot showed that nicotine (1 mmol/L) alone could not induce HSPs expression,but was able to enhance the HSP70 expression at 39 ℃ in RAW246.7.Immunofluorescence showed nicotine (1 mmol/L) was able to induce translocation of HSF1 from cytoplasm to nucleus,which was blocked by the pre-treating with hexamethonium apparently;EMSA showed that nicotine (1 mmol/L) could promote the binding of HSF1 to HSE at 37℃.Furthermore,nicotine (1 mmol/L) induced HSF1 binding to HSE greatly at 39℃.CONCLUSION:Nicotine (1 mmol/L) alone can induce HSF1 nuclear translocation and its DNA-binding activity to HSE,but not the expression of HSP70;nicotine (1 mmol/L) can enhance the expression of HSP70 under mild heat stress (39℃) through N1-receptor in the RAW264.7 macrophages.关键词
烟碱/热休克因子1/热休克蛋白70/N1受体Key words
nicotine/HSF1/HSP70/N1 receptor分类
医药卫生引用本文复制引用
谌崇峰,杨怀才,涂自智..烟碱通过N1受体促进热应激对RAW264.7细胞HSP70的诱导表达[J].中国临床药理学与治疗学,2017,22(2):124-131,8.
