中国临床药理学与治疗学2017，Vol.22Issue(2)：155-159,5.
HPV-DNA整合状态与宫颈癌发生发展及抑癌基因p53、pRb表达的相关性
Correlation between HPV-DNA integration and cervical cancer development as well as the expression of cancer suppressor gene p53 and pRb
摘要
Abstract
AIM:To analyze the correlation between HPV-DNA integration and cervical cancer development as well as the expression of cancer suppressor gene p53 and pRb,so as to provide new idea for its clinical diagnosis and treatment.METHODS:131 patients with cervical intraepithelial neoplasias (CIN) and cervical cancer which treated with surgery were selected and divided into 4 groups according to disease degree:CIN Ⅰ,CIN Ⅱ,CIN Ⅲ and cervical cancer;at the same time,33 patients with benign tumor were selected as controls.The pathological tissues were extracted and detected HPV-DNA integration using whole genome sequencing and high-throughput virus integrated experiment.According to HPV-DNA integration results,all the patients were divided into 3 groups:multi-integration,single-integration and non-integration,and the p53 and pRb expression levels between groups were compared.RESULTS:The HPV-DNA integration rate increased according to the healthy tissue,CIN Ⅰ,CIN Ⅱ,CIN Ⅲ,cervical cancer in the order,and there was mainly multi-integration in the cervical cancer,and mainly single-integration in CIN and the controls,the difference between groups had statistical significance (P ＜ 0.05).8 types of high-risk HPV-DNA integration,including HPV16,HPV58,HPV18,HPV35,HPV31,HPV33,HPV52 and HPV51 were found,with similar distribution trend in different disease degree.The p53 and pRb positive rate and expression level were different in different integration state,and non-integration group was higher than single-integration group,single-integration group was higher than multi-integration group,the difference between groups had statistical significance (P ＜ 0.05).CONCLUSION:HPV-DNA integration can restrain the expression of p53 and pRb and promote the development of cervical cancer,which may have a certain guiding role for the identification and prognosis evaluation of cervical cancer.关键词
人乳头瘤病毒/DNA整合/宫颈癌/抑癌基因Key words
human papilloma virus/DNA integration/cervical cancer/cancer suppressor gene分类
医药卫生引用本文复制引用
温川松,仝进毅..HPV-DNA整合状态与宫颈癌发生发展及抑癌基因p53、pRb表达的相关性[J].中国临床药理学与治疗学,2017,22(2):155-159,5.基金项目
浙江省自然科学基金项目(LY12H16018) （LY12H16018）
