中国临床医学2017，Vol.24Issue(1)：6-11,6.DOI:10.12025/j.issn.1008-6358.2017.20160524
升降散对脓毒症大鼠心肌p38 MAPK蛋白磷酸化水平的影响
Molecular mechanism of the protective effect of the Shensjianssan on myocardial injury induced by sepsis
摘要
Abstract
Objective:To study the molecular mechanism of myocardial inj ury induced by traditional Chinese medicine Shenhjianhsan.Methods:Male adult SD rats were randomly divided into normal group,model group,Shenhjianhsangroup and the p38 inhibitor group.By cecal ligation and puncture (CLP)of rat model of sepsis,the rat were observed at 4,8,12,and 24 h after operation.Shenhjianhsangroup and p38 inhibited rats respectively filled the stomach and subcutaneous injected the SB203580 in preoperative 2 h before givingShenhjianhsan.Blood samples from abdominal aortic were collected and heart tissue were detected.Observation and detection of each time node:mortality rate of rats,serum cTnI,BNP,IL-6 levels,myocardial p-p38MAPK protein,p-p38MAPK mRNA,IL-6 mRNA expression.Results:Model group has the 25%of mortality rates,while the Shenhjianhsangroup and the p38 inhibitor group both were 15%,which had no statistical significance.The cTnI and BNP were increased in model group,while the cTnI of 8 h,12 h and 24 h inShenhjianhsangroup were both improved (P<0.001), and the BNP of 4 h,12 h,and 24 h were all improved after BNP(P<0.05),but the difference betweenShenhjianhsangroup and the p38 inhibitor group has no statistical significance in every time node.The IL-6 level in the model group has been higher and while in theShenhjianhsangroup it has been decreased in every time node (P<0.001).The p-p38MARK protein level in model group grows higher,while the p-p38MARK protein level in every time node of Shenhjianhsangroup has improved(P<0.05).The expression of p-p38MARK mRNA has all improved in the 8 h,12 h,and 24 h ofShenhjianhsangroup (P<0.05). The cardiac muscle Il-6 mRNA has improved in model group,whileShenhjianhsangroup and p38 inhibitor group of 4 h,8 h, and 12 h has both improved (P<0.05),and the IL-6 mRNA become lower in the 12 h ofShenhjianhsangroup then of the p38 inhibitor group (P<0.05).Conclusions:Shenhjianhsancan effectively reduce serum cTnI and BNP of the early sepsis rat. The molecular mechanism of thatShenhjianhsanimproved myocardial injury in sepsis may be related to the regulation of sepsis early rat by reduction of the myocardial p-p38MAPK protein level and the expression of p-p38MAPK mRNA and IL-6 mRNA,and also related to the inhibition the excessive inflammatory reaction.关键词
脓毒症/心肌损伤/升降散/p38MAPK/炎症因子Key words
sepsis/myocardial injury/Shenhjianhsan/p38MAPK/inflammatory factors分类
医药卫生引用本文复制引用
赵雷,钱风华,丁纯蕾,王进,郭健..升降散对脓毒症大鼠心肌p38 MAPK蛋白磷酸化水平的影响[J].中国临床医学,2017,24(1):6-11,6.基金项目
国家自然科学基金青年基金(81303101),上海市卫生和计划生育委员会项目(201440399).Supported by National Natural Science Youth Foundation of China (81303101),and Shanghai Municipal Commission of Health and Family Planning Project(201440399). （81303101）
