中国临床医学2017，Vol.24Issue(1)：12-15,4.DOI:10.12025/j.issn.1008-6358.2017.20160913
Toonaciliatin A在体内外抑制胃癌MKN45细胞增殖并诱导细胞凋亡
Toonaciliatin A inhibits proliferation and induces apoptosis of gastric cancer MKN45 cells in vitro and in vivo
摘要
Abstract
Objective:To investigate the effects of traditional Chinese medicine monomer Toonaciliatin A on proliferation and apoptosis of human gastric cancer cell line MKN45invitroandinvivo.Methods:MKN45 cells were cultured invitroand treated with Toonaciliatin A (15,30,and 60μg/mL)for 48 h.Then the cell viability was measured by MTT assay.The apoptosis were determined by flow cytometry using Annexin V-FITC/PI assay.The BALB/c nude mouse xenograft model of MKN45 cells was established and administrated with Toonaciliatin A (10,20,and 40 mg/kg)for 21 days,then the weights of xenograft tumors and mouse were calculated,and the expression of caspase-3 protein in tumor tissues was detected by immunohistochemical method.Results:As compared with the control group,Toonaciliatin A inhibited effectively the proliferation of MKN45 cells,and induced apoptosis of MKN45 cellsinvitro(P<0.05).In the nude mouse xenograft model of MKN45 cells,the tumor weight of Toonaciliatin A treatment group was significantly decreased as compared with that of the control group (P<0.05 ).Toonaciliatin A induced the expression of caspase-3 proteininvivo (P<0.05 ).Conclusions:Toonaciliatin A is an effective anti-tumor Chinese medicine monomer.关键词
胃肿瘤/抗肿瘤药,植物/柠檬苦素类/异种移植模型抗肿瘤试验/ToonaciliatinAKey words
stomach neoplasms/antineoplastic/phytogenic/limonins/xenograft model antitumor assays/Toonaciliatin A分类
医药卫生引用本文复制引用
丁怡,卢新刚,孙书焰,戴俊..Toonaciliatin A在体内外抑制胃癌MKN45细胞增殖并诱导细胞凋亡[J].中国临床医学,2017,24(1):12-15,4.基金项目
上海市科学技术委员会课题(13401907000).Supported by Foundation of Shanghai Municipal Science and Technology Commission (13401907000). （13401907000）
