中国美容医学2017，Vol.26Issue(2)：5-8,4.
光动力学疗法对瘢痕成纤维细胞中转化生长因子β1的作用
The Investigation of Transforming Growth Factor β1 in HSF after HMME-PDT
摘要
Abstract
Objective To investigate the response of TGF-β1 after PDT which induced by hematoporphyrin monomerthyl ether (HMME) in human fibroblasts from hypertrophic scar (HSF). Methods Fibroblasts were cultured from nontreated hypertrophic scars, and cells were divided into 4 groups: control (untreated), HMME-PDT, HMME and Laser. The expression of TGF-β1 in supernatant of HSF was detected by enzyme linked immunosorbent assay (ELISA). The expression of TGF-β1was analyzed by Western blot. Results HMME-PDT down-regulated the protein level of TGF-β1 both in supernatant of HSF and in HSF.Conclusion HMME-PDT decreased TGF-β1 activation. It inhibited the proliferation of HSF through decreased the activation of taget gene.关键词
光动力学疗法/激光/光敏剂/瘢痕成纤维细胞/转化生长因子β1Key words
Photodynamic therapy/laser/Photosensitizer/hypertrophic scar fibroblast/TGF-β1分类
医药卫生引用本文复制引用
蔡宏,王毅侠,李铀,刘玮,孙平,董宁..光动力学疗法对瘢痕成纤维细胞中转化生长因子β1的作用[J].中国美容医学,2017,26(2):5-8,4.基金项目
本研究受国家自然科学基金资助(光动力学疗法作用于瘢痕疙瘩成纤维细胞 TGF-β1/Smad3 信号通路的分子机制:编号81301386) （光动力学疗法作用于瘢痕疙瘩成纤维细胞 TGF-β1/Smad3 信号通路的分子机制:编号81301386）
