中国免疫学杂志2017，Vol.33Issue(3)：338-342,5.DOI:10.3969/j.issn.1000-484X.2017.03.004
IgG型抗登革病毒NS1抗体介导被动系统性过敏反应的研究
IgG antibodies against Dengue virus non-structure protein 1 mediate passive sys-temic anaphylaxis in mice
摘要
Abstract
Objective:To ascertain whether the immune complexes (ICs) formed by Dengue virus 1 non-structure protein 1 (DENV1 NS1)and its IgG antibodies could mediate passive systemic anaphylaxis (PSA) and to explain the pathogenesis of Dengue hemorrhagic fever or Dengue shock syndrome (DHF/DSS).Methods:The monoclonal antibodies (mAbs) or mAb cocktails from 20 IgG mAbs of DENV1 NS1 prepared in this lab were screened to initiate PSA and passive cutaneous anaphylaxis (PCA) in mice.Meanwhile, the effects of GdCl3 and platelet activating factor ( PAF) antagonist CV-3988 on PSA induced by the NS1-IgG ICs were observed.Results:Two groups of monoclonal antibody cocktails with purified NS 1 were proved to be capable of provoking PCA and PSA in mice,whereas the other mAbs or mAb cocktails could be not .The murine PSA initiated by NS1-IgG(5D25+3B1) ICs could be sig-nificantly inhibited by in vivo treatment with GdCl3 or PAF antagonist CV-3988.Conclusion: The NS1-IgG ICs formed with DENV1 NS1 and IgG mAb cocktails can mediate PSA and PCA ,but not all of ICs formed by DENV 1 NS1 mAbs or mAb cocktails with DENV 1 NS1 can induce PSA ,indicating that it may be related to the special epitopes of DENV 1 NS1.The monocyte/macrophages and PAF may be as major effector cells and the major mediator for PSA induced by NS 1-IgG ICs,respectively.关键词
IgG/NS1/免疫复合物/被动系统性过敏反应(PSA)/登革休克综合征/被动皮肤过敏反应(PCA)Key words
IgG/NS1/Immune complexes/Passive systemic anaphylaxis ( PSA)/Dengue shock syndrome/Passive cutaneous分类
医药卫生引用本文复制引用
郭勇晖,朱伟,王艳芳,丁细霞,陈政良,富宁..IgG型抗登革病毒NS1抗体介导被动系统性过敏反应的研究[J].中国免疫学杂志,2017,33(3):338-342,5.基金项目
本文为国家自然科学基金委员会-广东省人民政府联合基金资助项目(U1132002). （U1132002）
