中国农业科技导报2017，Vol.19Issue(3)：131-136,6.DOI:10.13304/j.nykjdb.2016.622
响应曲面法优化猴头菇粗多糖的提取工艺
Optimization of Extraction Process for Crude Polysaccharide from Hericium Erinaceus by Response Surface Methodology
摘要
Abstract
Applying Box-Behnken design and response surface methodology,this study optimized the extraction conditions for crude polysaccharide from Hericium erinaceus investigation.The extraction time,ratio of water to raw material and extraction temperature were selected as the key investigation factors.Box-Behnken design took the extraction rate as the response value.Design Expert software 7.0 was used to establish quadratic multinomial models fitting for experimental data.Contour overlay plot and response surface were delineated for the comparison of interactions of each key factor.And then the optimal formulation was screened out and validated.The result showed that the optimal extraction parameters were as follows:extraction time 3.8 h,ratio of water to raw material 25 mL/g,extraction temperature 81℃.The results of validation test showed that the extraction rate was 3.09％,basically met with the forecast value.Research results showed the model established by the response surface methodology had good predictability,and could rationally optimize the extracting technology for crude polysaccharide from Hericium erinaceus.关键词
猴头菇粗多糖/响应曲面法/提取工艺Key words
crudepolysaccharide from Hericium erinaceus/response surface methodology/extraction technology分类
化学化工引用本文复制引用
陈新瑶,董星,陈景杰,秦韬,李健,黄一帆..响应曲面法优化猴头菇粗多糖的提取工艺[J].中国农业科技导报,2017,19(3):131-136,6.基金项目
国家自然科学基金项目(31502110)资助. （31502110）
