中国农业科学2017，Vol.50Issue(4)：669-678,10.DOI:10.3864/j.issn.0578-1752.2017.04.007
玉米大斑病菌Homeobox转录因子家族全基因组鉴定及其表达规律分析
Identification of Homeobox Transcription Factor Family in Genome-Wide and Expression Pattern Analysis of the Members inSetosphaeria turcica
摘要
Abstract
[Objective] The objectives of this study are to identify Homeobox transcription factor family fromSetosphaeria turcica genome, and to predict the locations of their corresponding coding proteins in the cells, gene structure and evolutionary characteristics, as well as to explore their expression patterns at different growth and development stages inS. turcica.[Method]Homeobox transcription factor family was identified based onS. turcica genome database and bioinformatics methods. Phylogenetic tree were created using the MEGA5.0 program. Gene structure characteristics were analyzed by GSDS software, which was a gene structure display server online (http://gsds1.cbi.pku.edu.cn/index.php). Amino acid sequence characteristics of the genes in Homeobox transcription factor family were compared using software Clustal X 1.83. The secondary structures of homeodomain proteins were predicted by employing software SOMPA. Three-dimensional structures of Homeobox transcription factor family were doped out using Software I-TASSER. Homeobox genes expression patterns were detected at different developmental stages based on quantitative real-time PCR (qRT-PCR) analysis.[Result] A total of 8 Homeobox genes were systematically identified fromS. turcica genome and classified into 4 groups according to the gene structure and phylogenetic tree. Subcellular localization showed that all of the predicted proteins located in the cell nuclei. The family members contained conserved Homeobox domains and typical "helix-turn-helix" secondary structures. Different gene family members took on different expression levels at different development stages in S. turcica.The expression level ofStHTF1was significantly higher at mycelium, spore and appressorium formation stages than other stages.StHTF3,StHTF4 had the highest expression level at spore formation stage than other stages inS.turcica, while StHTF6 was the highest at germ tube formation stage. Moreover,StHTF2,StHTF5,StHTF7 andStHTF8 appeared higher expression level at appressorium formation stage than other genes.[Conclusion] Homeobox transcription factor family inS. turcica contains 8 genes which are classed into 4 categories and located in the nuclei. The proteins encoded by the genes contain conserved Homeobox domain and "helix-turn-helix" spatial structure. Homeobox gene family members inS. turcicaappeareddiverse expression profiles which will lay the foundation for identifying the functions of the gene family.关键词
玉米大斑病菌/Homeobox转录因子家族/系统进化/基因表达Key words
Setosphaeria turcica/Homeobox transcription factor family/system evolution/gene expression引用本文复制引用
赵洁,赵立卿,巩校东,冯胜泽,刘星晨,郑亚男,李志勇,孙海月,王冬雪..玉米大斑病菌Homeobox转录因子家族全基因组鉴定及其表达规律分析[J].中国农业科学,2017,50(4):669-678,10.基金项目
国家自然科学基金(31271997,31371897)、大学生创新创业训练计划(201610086052,20161805) （31271997,31371897）
