中国农业科学2017，Vol.50Issue(4)：774-782,9.DOI:10.3864/j.issn.0578-1752.2017.04.017
马铃薯品种'青薯168'和'陇薯3号'块茎愈伤能力的比较
Comparison of Healing Ability on Potato Tuber Cultivars 'Qingshu No. 168' and 'Longshu No. 3'
摘要
Abstract
[Objective] The objective of this study is to find out the differences of tuber healing ability of resistant and susceptible potato cultivars against dry rot ('Qingshu No. 168' and 'Longshu No. 3'), and explore the reasons causing differences in the levels of lignin and suberin accumulation and phenylpropanoid metabolism.[Method] The resistant cultivar 'Qingshu No. 168' and susceptible cultivar 'Longshu No. 3' were used as materials. The damage of tubers was artificially simulated and heal was conducted at ambient temperature. The healing effect was assessed by determining the weight loss and disease index of wounded tubers that were inoculated withFusarium sulphureum at every healing time point. Phloroglucinol-HCl staining and toluidin blue O-neutral red staining were used to observe lignin and suberin deposition, and the lignified cell layers and suberin fluorescent intensity were quantified. Moreover, the key enzyme and metabolites of phenylpropanoid metabolism pathway such as phenylalanine ammonia lyase activity, and the contents of lignin, total phenol and flavonoid were determined to compare the differences of healing ability between the two cultivars.[Result]The weight loss of 'Qingshu No. 168' was significantly lower than that of 'Longshu No. 3' during healing. The weight loss of 'Qingshu No. 168' and 'Longshu No. 3' was 0.93% and 1.96% after 14 days of healing, and the weight loss of 'Qingshu No. 168' was 52.3% lower than that of 'Longshu No. 3'. The disease index of 'Qingshu No. 168' was also significantly lower than 'Longshu No. 3' after inoculated withF. sulphureum during healing. After 3 days of healing for inoculated tubers, the disease index of 'Qingshu No. 168' and 'Longshu No. 3' was 20.36 and 71.59, respectively, the former was 71.5% lower than the later. During healing, 'Qingshu No. 168' had higher accumulation rate of lignin and suberin than 'Longshu No. 3'. After healing for 14 days, the thickness of lignified cell layers and total fluorescent intensity of suberin of 'Qingshu No. 168' were 47.4% and 60.6% higher than 'Longshu No. 3'. In addition, 'Qingshu No. 168' showed more phenylpropanoid metabolism activity than 'Longshu No. 3'. The activity of phenylalanine ammonialyase and the lignin content of 'Qingshu No. 168' were 77% and 65% higher than 'Longshu No. 3'after healing for 14 days, respectively. The total phenolics contents of the two cultivars showed a significant difference at late stage of healing, 'Qingshu No. 168' was 76% higher than 'Longshu No. 3' after healing for 14 days. However, no significant difference was found in the flavonoids content between the two cultivars during healing.[Conclusion] The resistant cultivar 'Qingshu No. 168' showed stronger healing ability than susceptible cultivar 'Longshu No. 3'. A stronger healing ability of resistant cultivar is related with its higher phenylpropanoid metabolism activity.关键词
马铃薯/品种/块茎/愈伤/苯丙烷代谢Key words
potato/cultivars/tuber/wound healing/phenylpropanoid metabolism引用本文复制引用
姜红,毕阳,李昌健,王毅,李生娥,刘耀娜,王斌..马铃薯品种'青薯168'和'陇薯3号'块茎愈伤能力的比较[J].中国农业科学,2017,50(4):774-782,9.基金项目
国家自然科学基金(地区科学基金项目)(31460412)、甘肃农业大学盛彤笙科技创新基金(GSAU-STS-1432)、国家科技支撑计划(2012BAD06B00) （地区科学基金项目）
