中国全科医学2017，Vol.20Issue(9)：1055-1060,6.DOI:10.3969/j.issn.1007-9572.2017.09.007
当归挥发油对自发性高血压大鼠miR-155及其靶基因的影响
Effect of Angelica Naphtha on the Expression Levels of miR - 155 and Target Genes in Spontaneously Hypertensive Rats
摘要
Abstract
Objective To study the effect of angelica naphtha on the expression levels of miR - 155 and target genes in spontaneously hypertensive rats (SHR),and to investigate the influence and mechanism of action of angelica naphtha on lowering the blood pressure in SHR from the perspective of renin angiotensin aldosterone system (RAAS). Methods This study was conducted between October 2015 and April 2016. We randomized 48 SPF male SHR rats aged 8 weeks old into model group, low - dose angelica naphtha group,medium - dose angelica naphtha group,high - dose angelica naphtha group,ligustilide group,valsartan group with 8 SHR rats in each group,and selected 8 SPF male Wistar rats with normal blood pressure as the normal group. Low - dose angelica naphtha group,medium - dose angelica naphtha group,high - dose angelica naphtha group, ligustilide group,valsartan group were given intragastric administration of angelica naphtha emulsion 12. 6 mg·kg - 1 ·d - 1 , 37. 0 mg · kg - 1 · d - 1 ,100. 0 mg · kg - 1 · d - 1 ,ligustilide solution 0. 8 mg · kg - 1 · d - 1 ,valsartan solution 51. 4 mg·kg - 1 ·d - 1 ,respectively,once a day,for 4 weeks,while both the normal group and model group were not given any drugs during this period. All the groups had standard diet,and they could drink water freely during this period of intervention. Systolic blood pressure (SBP) was measured in each group before the intervention,at the 1st,2nd,3rd,and 4th weeks of intervention. All the rats were sacrificed and the heart was taken at the end of intervention. HE staining was used to recognize the morphologic changes in cardiac tissue. Western blotting was employed to determine the expression levels of angiotensin Ⅱ type 1 receptor (AT1R),extracellular signal - regulated kinase 1 / 2 (ERK1 / 2)and phosphorylated ERK 1 / 2 (p - ERK1 / 2). And RT - qPCR was adopted to measure the expression level of miR - 155. Results There was interaction on SBP in rats among different treatment ways and time (P < 0. 05). There was significant effect on SBP in rats among different treatment ways and time (P < 0. 05). Before the intervention,and at the 1st,2nd,3rd weeks of intervention,the SBP was lower in the normal group than in other groups (P < 0. 05). The 4th week of intervention,the SBP in model group was higher than nomal group (P< 0. 05). The SBP was lower in the model group than in other groups except the normal group before the intervention (P <0. 05),while it was higher in the model group than in other groups except the normal group at the 1st,2nd,3rd,4th weeks of intervention (P < 0. 05). HE staining showed that,the model group had unclear myocardial stripes with erythrocytes crowded in the cardiac tissue,which suggested that myocardial hyperemia and right ventricular dysfunction occurred in the group;low -dose,medium - dose,and high - dose angelica naphtha groups had clear myocardial stripes. The model group had higher expression levels of AT1R,ERK1 / 2,and p - ERK1 / 2 than low - dose,medium - dose,and high - dose angelica naphtha groups,ligustilide group,and valsartan group (P < 0. 05). The expression level of miR - 155 did not differ significantly between all the groups (P > 0. 05). Conclusion Angelica naphtha could significantly inhibit the expression of AT1R gene;by adjusting the ERK1 / 2 signaling pathway,it could lower the blood pressure;it did not affect the cardiac function via the expression of miR - 155.关键词
高血压/当归挥发油/miR-155/血管紧张素Ⅱ1型受体拮抗剂Key words
Hypertension/Angelica naphtha/miR - 155/Angiotensin Ⅱ type 1 receptor blockers分类
医药卫生引用本文复制引用
纪禄风,石向慧,王立红,伊琳..当归挥发油对自发性高血压大鼠miR-155及其靶基因的影响[J].中国全科医学,2017,20(9):1055-1060,6.基金项目
国家自然科学基金资助项目(81460669)——基于miRNA与RAS系统探讨当归降压的分子机制研究 （81460669）
甘肃省自然科学基金资助项目(1310RJZA084)——当归提取液对自发性高血压大鼠miRNA差异表达谱的影响 （1310RJZA084）
