中国水稻科学2017，Vol.31Issue(1)：23-30,8.DOI:10.16819/j.1001-7216.2017.6043
粳稻柱头外露率QTL定位
QTL Mapping of japonica Rice Stigma Exsertion Rate
摘要
Abstract
[Objective]Stigma exsertion is one of the important traits which contribute to the efficient improrement of commercial seed production in hybrid rice.The objective of the study was to understand the genetic factors involved in the sigma exsertion.[Method]The F2 and F2 :3 populations of Da S× D50 planted in Lingshui,Hainan Province and Hangzhou,Zhejiang Province,respectively were used and the single stigma exsertion rate (PSES),double stigma exsertion rate (PDES)and total stigma exsertion rate (PES)were measured.[Result]Correlation analysis indicated that there were significant relationships among the PSES,PDES and PES.Further,the genetic linkage map of Da S× D50 F2 population was constructed,then,21 stigma exsertion rate QTL were located on chromosome 1 ,2,3,4,6,7 and 12,respectively,with the contribution rate ranging from 0.1 % to 57.6%.The additive effect of most QTLs were contributed by high value parent Da S except for some QTL that with extremely low contribution rate.There were two QTLs on Chromosome 4 and Chromosome 12,qPDES4 and qPES12 ,both detected in F2 and F2 :3 populations with contribution rate as high as 17.86% and 16.98%,respectively.The qPES3 located on Chromosome 3 with high contribution rate were detected in F2 :3 population for two times.[Conclusion]Some stigma exsertion rate QTL shared the same location with those reported by other researchers,but there were also some new QTLs which provide theoretical support for further fine-mapping,gene cloning,and breeding new j aponica male sterile lines with high stigma exsertion rate by using molecular marker-assistant selection.关键词
柱头外露率/QTL定位/分子标记辅助选择(MAS)/粳稻Key words
stigma exsertion rate/QTL mapping/marker-assistant selection(MAS)/japonica rice分类
生物科学引用本文复制引用
李威,圣忠华,朱子亮,魏祥进,石磊,邬亚文,唐绍清,王建龙,胡培松..粳稻柱头外露率QTL定位[J].中国水稻科学,2017,31(1):23-30,8.基金项目
国家自然科学基金资助项目(31501285) （31501285）
浙江省自然科学基金资助项目(LY14C130009) （LY14C130009）
浙江省公益技术应用研究计划资助项目(2015C32045). （2015C32045）
